Heather Ruth Wolfe (born 1971) is an American curator of manuscripts and archivist at the Folger Shakespeare Library. A "Shakespeare detective", she has been noted for her research into the history of the Shakespeare coat of arms. She headed "Shakespeare Documented", a project to make contemporary texts involving Shakespeare available online, and is involved in the similar "Early Modern Manuscripts Online" (EMMO) project.
At the Folger, her "Project Dustbunny" has yielded significant results from human cells gathered from 17th-century volumes.