Whole-Body Regeneration Methods and Protocols /

This Open Access volume provides a comprehensive overview of the latest tools available to scientists to study the many facets of whole-body regeneration (WBR). The chapters in this book are organized into six parts. Part One provides a historical overview on the study of the WBR phenomena focusing...

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Bibliographic Details
Corporate Author: SpringerLink (Online service)
Other Authors: Blanchoud, Simon (Editor), Galliot, Brigitte (Editor)
Format: Electronic eBook
Language:English
Published: New York, NY : Springer US : Imprint: Humana, 2022.
Edition:1st ed. 2022.
Series:Methods in Molecular Biology, 2450
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Online Access:Link to Metadata
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Table of Contents:
  • The Hazards of Regeneration: From Morgan's Legacy to Evo-Devo
  • Studying Regeneration in Ascidians: An Historical Overview
  • Studying Protista WBR and Repair using Physarum polycephalum
  • Studying Porifera WBR using the Calcerous Sponges Leucosolenia
  • Studying Ctenophora WBR using Mnemiopsis leidyi
  • Studying Placozoa WBR in the Simplest Metazoan Animal, Trichoplax adhaerens
  • Collecting and Culturing Kamptozoans for Regenerative Studies
  • Collecting and Culturing Bryozoans for Regenerative Studies
  • Studying Annelida Regeneration in a Novel Model Organism: The Freshwater Aeolosoma viride
  • Studying Annelida Body Regeneration under Environmental Stress in Diopatra neapolitana
  • Studying Annelida Regeneration using Platynereis dumerilii
  • Collecting and Culturing Lineus sanguineus to Study Nemertea WBR
  • Studying Xenacoelomorpha WBR using Isodiametra pulchra
  • Studying Echinodermata Arm Explant Regeneration using Echinaster sepositus
  • Studying Hemichordata WBR using Ptychodera flava.-Studying Tunicata WBR using Botrylloides anceps
  • In Situ Hybridization to Identify Stem Cells in the Freshwater Sponge Ephydatia fluviatilis
  • Isolation and Maintenance of In Vitro Cell Cultures from the Ctenophore Mnemiopsis leidyi
  • Analysis of Spatial Gene Expression at the Cellular Level in Stony Corals
  • Studying Stem Cell Biology in Intact and Whole-Body Regenerating Hydra by Flow Cytometry
  • Non-Invasive Intra-Vascular Micro-Transfusion in Colonial Tunicates
  • Gene Manipulation in Hydractinia
  • Manipulation of Gene Activity in the Regenerative Model Sea Anemone, Nematostella vectensis
  • Monitoring Telomere Maintenance during Regeneration of Annelids
  • Analysis of DNA Double Stranded Breaks using the COMET Assay in Planarians
  • Random Integration Transgenesis in a Free-Living Regenerative Flatworm Macrostomum lignano
  • RNAi Screening to Assess Tissue Regeneration in Planarians
  • Monitoring Chromatin Regulation in Planarians using Chromatin Immunoprecipitation followed by Sequencing (ChIP-seq)
  • Assessing Chromatin Accessibility during WBR in Acoels
  • Single Cell Transcriptomic Analysis in the Regenerating Cnidarian Nematostella vectensis
  • Characterization of Soluble Cell-Free Coelomic Fluid Proteome from the Starfish Marthasterias glacialis
  • Using RNA-Sequencing for Transcriptome Profiling of Botrylloides sp. Regeneration
  • Studying Mechanical Oscillations during Whole-Body Regeneration in Hydra
  • Combining RNAi-Mediated β-catenin Inhibition and Reaggregation to Study Hydra Whole-Body Regeneration
  • Creating a User-Friendly and Open-Access Gene Expression Database for Comparing Embryonic Development and Regeneration in Nematostelle vectensis
  • Formalizing Phenotypes of Regeneration.