Low-Cost Methods for Molecular Characterization of Mutant Plants Tissue Desiccation, DNA Extraction and Mutation Discovery: Protocols /

• Main Authors: Till, Bradley J. (Author), Jankowicz-Cieslak, Joanna (Author), Huynh, Owen A. (Author), Beshir, Mayada M. (Author), Laport, Robert G. (Author), Hofinger, Bernhard J. (Author)
• מחבר תאגידי: SpringerLink (Online service)
• פורמט: אלקטרוני ספר אלקטרוני
• שפה: אנגלית
• יצא לאור: Cham : Springer International Publishing : Imprint: Springer, 2015.
• מהדורה: 1st ed. 2015.
• נושאים: Plant biotechnology.; Biology > Technique.; Biomaterials.; Nucleic acids.; Plant Biotechnology.; Biological Techniques.; Nucleic Acid.
• גישה מקוונת: Link to Metadata

סיכום

This book offers low-cost and rapid molecular assays for the characterization of mutant plant germplasm. Detailed protocols are provided for the desiccation of plant tissues; the extraction of high-quality DNA for downstream applications; the extraction of single-strand-specific nucleases for single nucleotide polymorphism; and small insertion/deletion discovery using standard agarose gel electrophoresis. The methods described can be applied in any laboratory equipped for basic molecular biology and do away with the need for expensive freezers and toxic organic compounds. With the appropriate validation of sample quality and longevity, they can provide sufficient DNA for a variety of molecular applications, such as marker studies and TILLING, at approximately one tenth of the cost per sample when compared to commercial kits.

תיאור פיזי

X, 35 p. 9 illus., 3 illus. in color. online resource.

ISBN

9783319162591

DOI

10.1007/978-3-319-16259-1

גישה

Open Access