Chemical Biology & Biochemistry Laboratory Using Genetic Code Expansion Manual
Chemical Biology & Biochemistry Laboratory Using Genetic Code Expansion Manual
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| Main Authors: | , , |
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| Format: | Electronic eBook |
| Language: | English |
| Published: |
Corvallis, Oregon
Oregon State University
2019.
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| Series: | Open textbook library.
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| Subjects: | |
| Online Access: | Access online version |
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Table of Contents:
- 1. Background
- 2. Overview of Laboratory Responsibilities Tentative Schedule
- 3. Objectives for Week 1
- 4. Concepts for Choosing and Expressing Mutants
- 5. Objectives for Week 2
- 6. Concepts for Protein Expression
- 7. Methods for Protein Expression
- 8. Concepts for SDS-PAGE
- 9. Methods for SDS-PAGE
- 10. Objectives for Week 3
- 11. Concepts for Protein Purification and Desalting
- 12. Methods for Protein Purification and Desalting
- 13. Microfluidizer Lysis Method
- 14. Objectives for Week 4
- 15. Methods for Protein Standard Curves and SDS-PAGE
- 16. Objectives for Weeks 5-9
- 17. Concepts for Enzyme Assays
- 18. Methods for Enzyme Assays