Microbial Biocatalysis

• Other Authors: Wang, Zhilong (Editor), Pan, Tao (Editor)
• Format: Electronic Book Chapter
• Language: English
• Published: Basel MDPI - Multidisciplinary Digital Publishing Institute 2023
• Subjects: Technology: general issues; Biotechnology; C058; mycelium biofloc; extracellular polymeric substances; microcosmic structure; microflora composition; acetophenone tolerance; whole-cell catalyst; directed evolution; (R)-1-phenylethanol; short-chain dehydrogenase/reductase; trans-cinnamic acid; p-coumaric acid; phenylalanine ammonia-lyase; whole-cell biotransformation; phenol; metabolites; whole genome sequencing; xenobiotics; atrazine; Arthrobacter sp. ST11; biodegradation; nonaqueous-phase liquid; cadmium; α-ketoglutaric acid biosynthesis; Yarrowia lipolytica; glycerol; rapeseed oil; culture conditions optimization; rabeprazole; sulfide; biotransformation; Cunninghamella blakesleeana 3.970; O-demethylation rabeprazole sulfide; microbial transformation; phytosterols; Pickering emulsion; phase inversion; plant oil; α-amylase; starch hydrolysis; marine enzymes; Persian Gulf; Streptomyces noursei; nysfungin; nystatin A1; nystatin A3; polyfungin B; UV mutagenesis; DHEA; C7-hydroxylation; Gibberella sp.; Absidia coerulea; biopolymers; aerobic composting; sustainability; depolymerization; enzymes; nitrilase; cell immobilization; nicotinic acid; reaction optimization; semi-continuous packed-bed bioreactor; diclofenac; immobilization; toxicity; sewage treatment; laccase; multifunctional enzymes; secondary metabolic; synthases; post-modifying enzymes; n/a
• Online Access: DOAB: download the publication; DOAB: description of the publication

Biocatalysis is a sustainable alternative for the chemical industry in manufacturing, monitoring, and waste management. Biocatalytic processes perform with isolated enzymes or whole cells as biocatalysts. Whole-cell biocatalysts offer some unique advantages of cascade reactions catalyzed by multienzymes as well as a single bioredox reaction with cofactor regeneration in a single strain. Therefore, whole-cell biocatalysts are widely applied for biosynthesis/biotransformation to produce value-added chemicals as well as the complete mineralization of organic pollutants.Biological catalytic processing using whole-cell biocatalysts includes biocatalyst engineering, bio-reaction engineering, and downstream processing. In addition to the traditional screening of microbial strains and immobilized whole-cell biocatalysts, modern genetic engineering, metabolic engineering, and synthetic biology make tailored whole-cell biocatalysts possible. At the same time, some integrated processes have successfully been applied in the catalytic processing using living whole-cell biocatalysts, such as harnessing biocompatible chemistry to interface with the microbial metabolism as well as using various separation techniques for in situ product removal.This reprint on "Microbial Biocatalysis" provides a comprehensive overview of the recent developments of catalyst discovery, catalyst modification, and process intensification for whole cell catalysis in fermentation, biotransformation or biodegradation processes.