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Advances in Single Molecule, Real-Time (SMRT) Sequencing

PacBio's single-molecule real-time (SMRT) sequencing technology offers important advantages over the short-read DNA sequencing technologies that currently dominate the market. This includes exceptionally long read lengths (20 kb or more), unparalleled consensus accuracy, and the ability to sequ...

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Detalles Bibliográficos
Autor principal: Ameur, Adam (auth)
Otros Autores: Hestand, Matthew S. (auth)
Formato: Electrónico Capítulo de libro
Lenguaje:inglés
Publicado: MDPI - Multidisciplinary Digital Publishing Institute 2019
Materias:
Biology, life sciences
n/a
Cladobotryum protrusum
allele-specific analysis
low-input DNA
full length RNAseq
de novo genome assembly
de novo assembly
human reference genome
Tricoplusia ni
PacBio single molecule real-time sequencing
secondary metabolite
protein isoforms
bone marrow cell subpopulations
DNA methylation
mycoparasite
human whole-genome sequencing
GRCh38
SMRT sequencing
cytochrome P450 enzyme (CYP)
mRNA isoforms
next generation sequencing
cobweb disease
Swedish population
mosquito
long-read SMRT sequencing
whole genome sequencing
terpenoid
insect genome
optical mapping
Gloeostereum incarnatum
population sequencing
statistical methods
gene expression
single molecule real-time sequencing
PacBio
Acceso en línea:DOAB: download the publication
DOAB: description of the publication
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Descripción
Sumario:PacBio's single-molecule real-time (SMRT) sequencing technology offers important advantages over the short-read DNA sequencing technologies that currently dominate the market. This includes exceptionally long read lengths (20 kb or more), unparalleled consensus accuracy, and the ability to sequence native, non-amplified DNA molecules. From fungi to insects to humans, long reads are now used to create highly accurate reference genomes by de novo assembly of genomic DNA and to obtain a comprehensive view of transcriptomes through the sequencing of full-length cDNAs. Besides reducing biases, sequencing native DNA also permits the direct measurement of DNA base modifications. Therefore, SMRT sequencing has become an attractive technology in many fields, such as agriculture, basic science, and medical research. The boundaries of SMRT sequencing are continuously being pushed by developments in bioinformatics and sample preparation. This book contains a collection of articles showcasing the latest developments and the breadth of applications enabled by SMRT sequencing technology.
Descripción Física:1 electronic resource (128 p.)
ISBN:books978-3-03921-701-4
9783039217014
9783039217007
Acceso:Open Access

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