Advances in Single Molecule, Real-Time (SMRT) Sequencing

• Main Author: Ameur, Adam (auth)
• Other Authors: Hestand, Matthew S. (auth)
• Format: Electronic Book Chapter
• Language: English
• Published: MDPI - Multidisciplinary Digital Publishing Institute 2019
• Subjects: Biology, life sciences; n/a; Cladobotryum protrusum; allele-specific analysis; low-input DNA; full length RNAseq; de novo genome assembly; de novo assembly; human reference genome; Tricoplusia ni; PacBio single molecule real-time sequencing; secondary metabolite; protein isoforms; bone marrow cell subpopulations; DNA methylation; mycoparasite; human whole-genome sequencing; GRCh38; SMRT sequencing; cytochrome P450 enzyme (CYP); mRNA isoforms; next generation sequencing; cobweb disease; Swedish population; mosquito; long-read SMRT sequencing; whole genome sequencing; terpenoid; insect genome; optical mapping; Gloeostereum incarnatum; population sequencing; statistical methods; gene expression; single molecule real-time sequencing; PacBio
• Online Access: DOAB: download the publication; DOAB: description of the publication

PacBio's single-molecule real-time (SMRT) sequencing technology offers important advantages over the short-read DNA sequencing technologies that currently dominate the market. This includes exceptionally long read lengths (20 kb or more), unparalleled consensus accuracy, and the ability to sequence native, non-amplified DNA molecules. From fungi to insects to humans, long reads are now used to create highly accurate reference genomes by de novo assembly of genomic DNA and to obtain a comprehensive view of transcriptomes through the sequencing of full-length cDNAs. Besides reducing biases, sequencing native DNA also permits the direct measurement of DNA base modifications. Therefore, SMRT sequencing has become an attractive technology in many fields, such as agriculture, basic science, and medical research. The boundaries of SMRT sequencing are continuously being pushed by developments in bioinformatics and sample preparation. This book contains a collection of articles showcasing the latest developments and the breadth of applications enabled by SMRT sequencing technology.