Advances in Single Molecule, Real-Time (SMRT) Sequencing

• Main Author: Ameur, Adam (auth)
• Other Authors: Hestand, Matthew S. (auth)
• Format: Electronic Book Chapter
• Language: English
• Published: MDPI - Multidisciplinary Digital Publishing Institute 2019
• Subjects: Biology, life sciences; n/a; Cladobotryum protrusum; allele-specific analysis; low-input DNA; full length RNAseq; de novo genome assembly; de novo assembly; human reference genome; Tricoplusia ni; PacBio single molecule real-time sequencing; secondary metabolite; protein isoforms; bone marrow cell subpopulations; DNA methylation; mycoparasite; human whole-genome sequencing; GRCh38; SMRT sequencing; cytochrome P450 enzyme (CYP); mRNA isoforms; next generation sequencing; cobweb disease; Swedish population; mosquito; long-read SMRT sequencing; whole genome sequencing; terpenoid; insect genome; optical mapping; Gloeostereum incarnatum; population sequencing; statistical methods; gene expression; single molecule real-time sequencing; PacBio
• Online Access: DOAB: download the publication; DOAB: description of the publication

PacBio's single-molecule real-time (SMRT) sequencing technology offers important advantages over the short-read DNA sequencing technologies that currently dominate the market. This includes exceptionally long read lengths (20 kb or more), unparalleled consensus accuracy, and the ability to sequ...