Konzeption und prototypische Fertigung einer nicht-invasiven mikrofluidischen Plattform für die Elektrophysiologie (NIMEP) zur Zellenanalyse
The most commonly used measurement technique for electrophysiology is the patch clamp technique. While this measurement technique allows the precise investigation of the communication taking place through ion channels, it has some undesirable drawbacks such as the local destruction of the plasma mem...
Saved in:
| Main Author: | |
|---|---|
| Format: | Electronic Book Chapter |
| Published: |
KIT Scientific Publishing
2015
|
| Series: | Schriften des Instituts für Mikrostrukturtechnik am Karlsruher Institut für Technologie / Hrsg.: Institut für Mikrostrukturtechnik
|
| Subjects: | |
| Online Access: | DOAB: download the publication DOAB: description of the publication |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| Summary: | The most commonly used measurement technique for electrophysiology is the patch clamp technique. While this measurement technique allows the precise investigation of the communication taking place through ion channels, it has some undesirable drawbacks such as the local destruction of the plasma membrane, a low success rate and an elaborate experimental procedure. To avoid these drawbacks, in this work a new non-invasive microfluidic platform for electrophysiological research (NIMEP) was developed with regard to the activity of ion channels. This novel approach is based on the non-invasive measurement of the total current through the cell membrane and provides a possibility for an automated investigation of the individual cells. In addition, the investigated cell can be used for other applications, since the cell remains in an intact state before and after the test. |
|---|---|
| Physical Description: | 1 electronic resource (XVII, 171 p. p.) |
| ISBN: | KSP/1000046877 9783731503781 |
| Access: | Open Access |