Fluorescence Methods for Investigation of Living Cells and Microorganisms
Fluorescence methods play a leading role in the investigation of biological objects. They are the only non-destructive methods for investigating living cells and microorganisms in vivo. Using intrinsic and artificial fluorescence methods provides deep insight into mechanisms underlying physiological...
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| Format: | Elektronički Poglavlje knjige |
| Jezik: | engleski |
| Izdano: |
IntechOpen
2020
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| Teme: | |
| Online pristup: | DOAB: download the publication DOAB: description of the publication |
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| Sažetak: | Fluorescence methods play a leading role in the investigation of biological objects. They are the only non-destructive methods for investigating living cells and microorganisms in vivo. Using intrinsic and artificial fluorescence methods provides deep insight into mechanisms underlying physiological and biochemical processes. This book covers a wide range of modern methods involved in experimental biology. It illustrates the use of fluorescence microscopy and spectroscopy, confocal laser scanning microscopy, flow cytometry, delayed fluorescence, pulse-amplitude-modulation fluorometry, and fluorescent dye staining protocols. This book provides an overview of practical and theoretical aspects of fluorescence methods and their successful application in the investigation of static and dynamic processes in living cells and microorganisms. |
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| Opis fizičkog objekta: | 1 electronic resource (462 p.) |
| ISBN: | intechopen.83296 9781839680403 9781839680397 9781839680410 |
| Pristup: | Open Access |