Peptide Nucleic Acids Applications in Biomedical Sciences

• Other Authors: Yavin, Eylon (Editor)
• Format: Electronic Book Chapter
• Language: English
• Published: Basel, Switzerland MDPI - Multidisciplinary Digital Publishing Institute 2020
• Subjects: Medicine; RNA structure; strand invasion; antisense; PNA; exon skipping; exon inclusion; oligonucleotides; peptide nucleic acid (PNA); antibacterials; RNA; PNA transporters; conjugates; bacterial resistance; peptide nucleic acids; triplex; gene editing; structure; recombination; repair; nanoparticles; β-thalassemia; cystic fibrosis; peptide nucleic acid; tolane; single nucleotide polymorphism; influenza virus; drug resistance; peptide nucleic acids (PNAs); single-nucleotide polymorphism (SNP); polymerase chain reaction (PCR); cancer.; catalytic hairpin assembly (CHA); strand-displacement reaction; l-DNA; microRNA; fluorescence; templated reactions; light-triggered; electrochemical biosensors; colorimetric detection; Peptide nucleic acids; PNA-masking; microRNAs; miR-145-5p; miRNA targeting; delivery; CFTR; n/a
• Online Access: DOAB: download the publication; DOAB: description of the publication

This book compiles four review articles and four research papers that highlight recent developments in the field of peptide nucleic acid (PNA) chemistry and biomedicine. The review articles encompass a variety of fields related to PNA, emphasizing the versatility of this DNA mimic. Two excellent reviews detail the use of PNA for molecular diagnostics of miRNAs and genetic point mutations (SNPs). Another review provides a comprehensive analysis of the various approaches for gene editing using chemically modified PNA molecules. Lastly, PNA molecules are elegantly described as effective (antisense) antimicrobial agents in the final review. The high binding affinity of PNA to complementary DNA and RNA is highlighted in three research articles. Two articles show how PNA molecules act as splice modulating and RNA masking molecules, separately. In another contribution, the high affinity and achiral characteristics of PNAs are used to developed a stable L-DNA-based catalytic hairpin assembly. Lastly, chemically-modified PNA molecules are shown to be superior probes for SNP detection. Altogether, these studies illustrate how PNA molecules may be useful for a variety of biomedical applications as either therapeutic or diagnostic agents.