Immunophenotyping in Autoimmune Diseases and Cancer

• Other Authors: Szebeni, Gábor J. (Editor), Puskás, László G. (Editor)
• Format: Electronic Book Chapter
• Language: English
• Published: Basel, Switzerland MDPI - Multidisciplinary Digital Publishing Institute 2020
• Subjects: Medicine; CD8+CD28− T cells; cancer immunology; glioblastoma; immunotherapy; malignant glioma; cancer; accidental cell death; oncosis; DDR; parthanatos; flow cytometry; systemic lupus erythematosus; T cells; glycosylation; sialylation; lectin binding; glycosylation enzymes; galectin 1; choriocarcinoma; hydatidiform mole; galectin; gestational trophoblastic disease; placental-specific gene; systems biology; trophoblast differentiation; B cells; non-switched B cells; systemic sclerosis; dcSSc; TLR; CD180; RP105; CpG; IL-6; IL-10; natural autoantibodies; IgM; citrate synthase; DNA topoisomerase I; unfolded protein response; Inositol-requiring enzyme 1 (IRE1); PKR-like endoplasmic reticulum kinase (PERK); Glucose-regulated protein 78 (GRP78); Activating transcription factor 6 (ATF6); immune cells; T cell; macrophage; tumor microenvironment; single cell mass cytometry; metastatic breast cancer; myeloid-derived suppressor cells; immunophenotyping; breast cancer; trastuzumab; chimeric antigen receptor; cell therapy; neuroendocrine neoplasia; neuroendocrine tumor; neuroendocrine carcinoma; immunohistochemistry; syntaxin 1
• Online Access: DOAB: download the publication; DOAB: description of the publication

The cooperation of highly specialized cell types maintains the homeostasis of multicellular organisms. The disturbance of that harmony contributes to the development of several diseases. Most of the cellular functions are executed by proteins, so it is essential to investigate biological processes at the protein level. Antibodies, complex biomolecules with high specificity, are used to recognize our protein of interest in a process known as "immunophenotyping". One of the routinely used methods to study cellular proteins is flow cytometry, which detects cell surface or intracellular proteins at single-cell resolution. The other most frequent technique is the traditional immunohistochemical investigation of microscopic sections of human tissues. We called authors to publish their latest data studying cancer or autoimmune diseases by immunophenotyping.