Plant Cryopreservation

• Other Authors: Benelli, Carla (Editor)
• Format: Electronic Book Chapter
• Language: English
• Published: Basel MDPI - Multidisciplinary Digital Publishing Institute 2022
• Subjects: Research & information: general; activated charcoal; alginate; cryopreservation; European chestnut; zygotic embryo; apical shoot tips; axillary shoot tips; droplet-vitrification; long-term conservation; PVS2 vitrification; V cryo-plate; cryoprotectant distribution; raman microscopy; vitrification; ex situ conservation; embryogenic; shoot tips; plant biodiversity; currants; cryobanking; plant genebanks; plant genetic resources; germplasm collections; liquid nitrogen; long-term preservation; safety back-ups; conservation; endangered species; micropropagation; shoot culture; Xerophyllum asphodeloides; Eastern Turkey Beard; Symbiodiniaceae; two-step freezing; protein expression; coral; cryoprotectant; differential scanning calorimetry; glass transition; water crystallization; dormant buds; low-temperature resistance; pear tree; ultra-low temperature; glassy state; toxicity; n/a
• Online Access: DOAB: download the publication; DOAB: description of the publication

Cryopreservation is the storage of cells, tissues and organs at ultra-low temperatures (−196 °C) and can be an important tool for long-term conservation of plant genetic resources. A wide range of cryogenic procedures have been developed for in vitro explants, dormant buds and non-orthodox seeds in many plant species; however, research that provides novelty, insight and advances for developing and improving new protocols in plant species is still needed. This book includes the development of new protocols, physical and chemical studies related to preservation in liquid nitrogen, and some reviews on several aspects of cryopreservation. This publication will be a useful document for the community of cryopreservation scientists.