Efecto antibacterial in vitro del extracto etanolico de Hibiscus sabdariffa L. sobre cepas de Streptococcus mutans
Dental caries is one of the most frequent diseases worldwide and due to its prevalence, phytotherapy began to be used as an alternative treatment. Hibiscus sabdariffa L. has a wide variety of therapeutic properties, of which its antibacterial activity stands out in this study. Objective: The objecti...
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Language: | Spanish |
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Instituto Universitario de Innovación Ciencia y Tecnología Inudi Perú
2022
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100 | 1 | |a Padilla, Luis |4 auth | |
700 | 1 | |a Neyra, Kamyre |4 auth | |
700 | 1 | |a Huacasi, Vladimir |4 auth | |
700 | 1 | |a Macedo, Sonia |4 auth | |
700 | 1 | |a Huacasi, Vladimir |4 edt | |
700 | 1 | |a Huacasi, Vladimir |4 oth | |
245 | 1 | 0 | |a Efecto antibacterial in vitro del extracto etanolico de Hibiscus sabdariffa L. sobre cepas de Streptococcus mutans |
260 | |a Puno |b Instituto Universitario de Innovación Ciencia y Tecnología Inudi Perú |c 2022 | ||
300 | |a 1 electronic resource (84 p.) | ||
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506 | 0 | |a Open Access |2 star |f Unrestricted online access | |
520 | |a Dental caries is one of the most frequent diseases worldwide and due to its prevalence, phytotherapy began to be used as an alternative treatment. Hibiscus sabdariffa L. has a wide variety of therapeutic properties, of which its antibacterial activity stands out in this study. Objective: The objective of this research was to determine the in vitro antibacterial effect of the ethanolic extract of Hibiscus sabdariffa L. on strains of Streptococcus mutans at 24 and 48 hours. Materials and methods: The research was applied to experimental design and prospective measurement. The sample was obtained from patients who signed the informed consent in order to obtain the Streptococcus mutans strains. They were cultivated in 16 Petri dishes and 7 discs of filter paper No. 4 were distributed in each plate, the technique was Kirby Bauer disc diffusion with wells, with a total of 112 discs and wells; the Petri dishes were divided into 4 groups according to the concentrations of the ethanolic extract of Hibiscus sabdariffa L. that was obtained by maceration with 96% alcohol to obtain a sample of 100% ethanolic extract of Hibiscus sabdariffa L. and to obtain the concentrations of 25%, 50% and 75% alcohol was used to make the dilutions. Chlorhexidine 0.12% was considered as positive control and distilled water as negative control. Results: It was shown that Hibiscus sabdariffa L. has an inhibitory effect. The best effect was recorded at 48 hours at 100% concentration. Obtaining an inhibition halo of 14.23mm. Conclusion: The ethanolic extract of Hibiscus sabdariffa L. does have an in vitro antibacterial effect on Streptococcus mutans strains. | ||
520 | |a La caries dental es una de las enfermedades más frecuentes a nivel mundial y debido a su prevalecía se comenzó a utilizar la fitoterapia como tratamiento alternativo, la Hibiscus sabdariffa L. posee gran variedad de propiedades terapéuticas de las cuales en este estudio destaca su actividad antibacterial. Objetivo: El objetivo de esta investigación fue determinar el efecto antibacterial in vitro del extracto etanólico de Hibiscus sabdariffa L. sobre cepas de Streptococcus mutans a las 24 y 48 horas. Materiales y métodos: La investigación fue aplicativa de diseño experimental y medición prospectiva. La muestra fue obtenida de pacientes que firmaron el consentimiento informado para así poder obtener las cepas de Streptococcus mutans. Se cultivaron en 16 placas Petri y en cada placa se distribuyeron 7 discos de papel filtro N°4, la técnica fue de difusión por disco de Kirby Bauer con pozos, con un total de 112 discos y pozos; las placas Petri se dividieron en 4 grupos de acuerdo a las concentraciones del extracto etanólico de Hibiscus sabdariffa L. que se obtuvo por maceración con alcohol al 96% para obtener una muestra al 100% extracto etanólico de Hibiscus sabdariffa L. y para obtener las concentraciones de 25%, 50% y 75% se utilizó alcohol para realizar las diluciones. Se consideró la clorhexidina al 0.12% como control positivo y al agua destilada como control negativo. Resultados: Sé demostró que la Hibiscus sabdariffa L. tiene efecto inhibitorio. El mejor efecto se registró a las 48 horas al 100% de concentración. Obteniendo un halo de inhibición de 14.23mm. Conclusión: El extracto etanólico de la Hibiscus sabdariffa L. sí tiene efecto antibacterial in vitro sobre las cepas de Streptococcus mutans. | ||
540 | |a Creative Commons |f https://creativecommons.org/licenses/by/4.0/ |2 cc |4 https://creativecommons.org/licenses/by/4.0/ | ||
546 | |a Spanish | ||
650 | 7 | |a Oral & maxillofacial surgery |2 bicssc | |
653 | |a extracto etanólico | ||
653 | |a hibiscus sabdariffa L. | ||
653 | |a streptococcus mutans | ||
856 | 4 | 0 | |a www.oapen.org |u https://editorial.inudi.edu.pe/index.php/editorialinudi/catalog/book/13 |7 0 |z DOAB: download the publication |
856 | 4 | 0 | |a www.oapen.org |u https://directory.doabooks.org/handle/20.500.12854/92188 |7 0 |z DOAB: description of the publication |