Pressure Perturbation Approach in Biochemistry and Structural Biology. In memoriam of Dr. Gaston Hui Bon Hoa

• Other Authors: Davydov, Dmitri (Editor), Jung, Christiane (Editor)
• Format: Electronic Book Chapter
• Language: English
• Published: Basel MDPI - Multidisciplinary Digital Publishing Institute 2022
• Subjects: Research & information: general; Biology, life sciences; Biochemistry; protein folding; NMR; high hydrostatic pressure; thermodynamic stability; protein-ligand binding; high pressure; Martian salts; perchlorate; BSA; ANS; viroid; hydrostatic pressure; temperature; structure-activity relationship; RNA World; n/a; G-quadruplex; i-motif; volumetric properties; pressure-temperature phase diagram; thermodynamics; hepatitis B; DNA; oligo; FRET; FTIR; spectroscopy; pressure; volume change; TMPyP4; deep-sea adaptations; compressibility; cavities; potential energy landscape; yeast; Saccharomyces cerevisiae; high-pressure response; genetic manipulation; transcriptomics; piezophysiology; Anfinsen's dogma; native state N; unfolded state U; fibril state F; protofibrils; hen lysozyme; circular dichroism; 1H NMR spectroscopy; atomic force microscopy; cytochrome P450 reductase; conformational change; pressure-perturbation spectroscopy; protein hydration; reduction kinetics; stop-flow spectroscopy; Sorghum bicolor
• Online Access: DOAB: download the publication; DOAB: description of the publication

This Special Issue focuses on the effects of hydrostatic pressure on biological systems and the use of these effects for exploring the structure, function, and molecular dynamics of biological macromolecules and their ensembles. Here, we present a selection of papers highlighting new experimental findings and new theoretical concepts in high-pressure biosciences. In these studies, the authors combine pressure perturbation approaches with NMR and optical spectroscopy, kinetic and thermodynamic techniques, functional genomics and transcriptomics, and molecular dynamics simulations to gain new insights into the conformational dynamics of proteins and nucleic acids and to better understand the mechanisms of high-pressure adaptation in piezophiles. The articles collected in this issue demonstrate the unique exploratory potential of the pressure perturbation approach for biochemistry, biophysics, mechanistic enzymology, and evolutionary biology.