Strategies for CRISPR/Cas9-mediated genome editing: from delivery to production of modified plants

Strategies for CRISPR/Cas9-mediated genome editing: from delivery to production of modified plants

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Despite the conspicuous and rapid development of genome editing tools, implementing this technology in plants often remains constrained by our inability to regenerate fertile genome-modified plants. Finding a viable combination of delivery method, genome editing reagents, and plant regeneration syst...

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Bibliographic Details
Main Author: Gordon-Kamm, William (auth)
Other Authors: Barone, Pierluigi (auth), Svitashev, Sergei (auth), D. Sander, Jeffry (auth), Kumar, Sandeep (auth), Jones, Todd (auth)
Format: Electronic Book Chapter
Language:English
Published: Cambridge Burleigh Dodds Science Publishing 2021
Series:Burleigh Dodds Series in Agricultural Science
Subjects:
Agronomy & crop production
Sustainable agriculture
particle bombardment
electroporation
agrobacterium
Viral-based replicons
nanoparticles
Online Access:DOAB: download the publication
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Summary:Despite the conspicuous and rapid development of genome editing tools, implementing this technology in plants often remains constrained by our inability to regenerate fertile genome-modified plants. Finding a viable combination of delivery method, genome editing reagents, and plant regeneration system has often been both crop-specific and crop-limited. Recent progress using morphogenic genes such as Wuschel2 (Wus2) and Babyboom (Bbm) has alleviated some of these crop-specific challenges, and along with other improvements, continues to expand the range of plants that can be edited.
Physical Description:1 electronic resource (36 p.)
ISBN:AS.2020.0082.12
9781786768636
Access:Open Access

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