Strategies for CRISPR/Cas9-mediated genome editing: from delivery to production of modified plants

Despite the conspicuous and rapid development of genome editing tools, implementing this technology in plants often remains constrained by our inability to regenerate fertile genome-modified plants. Finding a viable combination of delivery method, genome editing reagents, and plant regeneration syst...

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Bibliographic Details
Main Author: Gordon-Kamm, William (auth)
Other Authors: Barone, Pierluigi (auth), Svitashev, Sergei (auth), D. Sander, Jeffry (auth), Kumar, Sandeep (auth), Jones, Todd (auth)
Format: Electronic Book Chapter
Language:English
Published: Cambridge Burleigh Dodds Science Publishing 2021
Series:Burleigh Dodds Series in Agricultural Science
Subjects:
Online Access:DOAB: download the publication
DOAB: description of the publication
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520 |a Despite the conspicuous and rapid development of genome editing tools, implementing this technology in plants often remains constrained by our inability to regenerate fertile genome-modified plants. Finding a viable combination of delivery method, genome editing reagents, and plant regeneration system has often been both crop-specific and crop-limited. Recent progress using morphogenic genes such as Wuschel2 (Wus2) and Babyboom (Bbm) has alleviated some of these crop-specific challenges, and along with other improvements, continues to expand the range of plants that can be edited. 
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