Solid-Phase Microextraction and Related Techniques in Bioanalysis

• Other Authors: Kataoka, Hiroyuki (Editor)
• Format: Electronic Book Chapter
• Language: English
• Published: Basel MDPI - Multidisciplinary Digital Publishing Institute 2023
• Subjects: Research & information: general; Chemistry; Analytical chemistry; tobacco-specific nitrosamines; hair; exposure biomarkers; in-tube solid-phase microextraction (SPME); liquid chromatography-tandem mass spectrometry (LC-MS/MS); bioanalysis; carbon materials; HPLC-MS; biological matrices; solid-phase extraction; sample preparation; bladder cancer (BC); metabolomics; solid phase microextraction (SPME); liquid chromatography; mass spectrometry; urine; Myrtaceae; native fruit; volatile compounds; sesquiterpenes; body odor; 2-nonenal; wiping method; pasting method; solid-phase microextraction (HS-SPME); gas chromatography-mass spectrometry (GC-MS); passive smoking; nicotine; cotinine; risk assessment; headspace solid-phase microextraction; Cerrado; aroma; volatile organic compounds; Herpetospermum pedunculosum; HS-SPME-GC-MS; dioecious plant; bud; volatile compound; multivariate statistical analysis; green peach aphids; VOCs; parasitoids; Aphidus colemani; Aphelinus abdominalis; cabbage; cerrado; Anacardiaceae; aroeira; biological properties; virtual screening; chemoinformatics; volatile organic compounds (VOCs); headspace solid-phase microextraction (HS-SPME); SPME-GC-MS; stored-product insect pests; Tribolium castaneum; Rhyzopertha dominica; Sitophilus granarius; titanium wire; superhydrophobic; superhydrophilic; in-tube solid-phase microextraction; high-performance liquid chromatography; sulfated steroid metabolites; saliva; online automated analysis; in-tube solid-phase microextraction (IT-SPME); stable isotope dilution; human body odor; GO:PANI/ZNRs/ZIF−8; response surface methodology; needle-based adsorbent; headspace-in needle microextraction; n/a
• Online Access: DOAB: download the publication; DOAB: description of the publication

Bioanalysis of endogenous substances, metabolites, and contaminants poisons is important in analyses of biological functions, metabolomics, forensic toxicology, patient diagnosis and biomonitoring of human exposure to hazardous chemicals. In these analyses, methods of sample preparation are essential for the isolation and concentration of target analytes from complex biological matrices. These processes, however, are time-consuming, labor-intensive and error-prone, and markedly influence the reliability and accuracy of determining target analytes. Thus, efficient sample preparation techniques and their integration with analytical methods have become significant. Solid-phase microextraction (SPME) is a simple and convenient sample preparation technique that has enabled automation, miniaturization, high-throughput performance, and online coupling with analytical instruments. Moreover, SPME has reduced analysis times, as well as solvent and disposal costs.This reprint consists of 14 original, peer-reviewed papers for the Special Issue in the MDPI journal Molecules. The topics covered include headspace fiber SPME (HS-SPME) gas chromatography-mass spectrometry (GC-MS), HS-in-needle microextraction GC-MS, thin film SPME liquid chromatography-tandem mass spectrometry (LC-MS/MS), magnetic solid phase extraction LC-MS/MS, in-tube SPME LC-MS/MS and in-tube SPME LC-UV. Samples analyzed include plant-derived volatile organic compounds; body odor; metabolites in urine, plasma and saliva sample; and biomarkers of tobacco smoke exposure in hair and environmental estrogens.