DNA flow cytometric analysis in variable types of hydropic placentas

• Główni autorzy: Fatemeh Atabaki pasdar (Autor), Alireza Khooei (Autor), Alireza Fazel (Autor), Maryam Rastin (Autor), Nafise Tabasi (Autor), Tahmineh Peirouvi (Autor), Mahmoud Mahmoudi (Autor)
• Format: Książka
• Wydane: Shahid Sadoughi University of Medical Science, Yazd, Iran, 2015-05-01T00:00:00Z.
• Hasła przedmiotowe: article
• Dostęp online: Connect to this object online.
• Notes: 1680-6433; 1680-6433
• Keywords: Gynecology and obstetrics; RG1-991; Reproduction; QH471-489
• Source: Iranian Journal of Reproductive Medicine, Vol 13, Iss 5, Pp 269-274 (2015)

Background: Differential diagnosis between complete hydatidiform mole, partial hydatidiform mole and hydropic abortion, known as hydropic placentas is still a challenge for pathologists but it is very important for patient management. Objective: We analyzed the nuclear DNA content of various types of hydropic placentas by flowcytometry. Materials and Methods: DNA ploidy analysis was performed in 20 non-molar (hydropic and non-hydropic spontaneous abortions) and 20 molar (complete and partial moles), formalin-fixed, paraffin-embedded tissue samples by flow cytometry. The criteria for selection were based on the histopathologic diagnosis. Results: Of 10 cases histologically diagnosed as complete hydatiform mole, 9 cases yielded diploid histograms, and 1 case was tetraploid. Of 10 partial hydatidiform moles, 8 were triploid and 2 were diploid. All of 20 cases diagnosed as spontaneous abortions (hydropic and non-hydropic) yielded diploid histograms. Conclusion: These findings signify the importance of the combined use of conventional histology and ploidy analysis in the differential diagnosis of complete hydatidiform mole, partial hydatidiform mole and hydropic abortion.