mNGS-based dynamic pathogen monitoring for accurate diagnosis and treatment of severe pneumonia caused by fungal infections
Metagenomic next-generation sequencing (mNGS) has been widely applied to identify pathogens associated with infectious diseases. However, limited studies have explored the use of mNGS-based dynamic pathogen monitoring in intensive care unit patients with severe pneumonia. Here, we present a clinical...
Saved in:
| Main Authors: | , , , , , , , , |
|---|---|
| Format: | Book |
| Published: |
Elsevier,
2023-06-01T00:00:00Z.
|
| Subjects: | |
| Online Access: | Connect to this object online. |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
MARC
| LEADER | 00000 am a22000003u 4500 | ||
|---|---|---|---|
| 001 | doaj_004ad39d3a9b43d4be85a97eb76111d6 | ||
| 042 | |a dc | ||
| 100 | 1 | 0 | |a Zhen Li |e author |
| 700 | 1 | 0 | |a Changcheng Wu |e author |
| 700 | 1 | 0 | |a Li-An Tang |e author |
| 700 | 1 | 0 | |a Yinjie Liang |e author |
| 700 | 1 | 0 | |a Ruhan A |e author |
| 700 | 1 | 0 | |a Debin Huang |e author |
| 700 | 1 | 0 | |a Chuanyi Ning |e author |
| 700 | 1 | 0 | |a Wenling Wang |e author |
| 700 | 1 | 0 | |a Wenjie Tan |e author |
| 245 | 0 | 0 | |a mNGS-based dynamic pathogen monitoring for accurate diagnosis and treatment of severe pneumonia caused by fungal infections |
| 260 | |b Elsevier, |c 2023-06-01T00:00:00Z. | ||
| 500 | |a 2590-0536 | ||
| 500 | |a 10.1016/j.bsheal.2023.04.004 | ||
| 520 | |a Metagenomic next-generation sequencing (mNGS) has been widely applied to identify pathogens associated with infectious diseases. However, limited studies have explored the use of mNGS-based dynamic pathogen monitoring in intensive care unit patients with severe pneumonia. Here, we present a clinical case of an 86-year-old male patient with severe pneumonia caused by a fungal infection. During the clinical treatment, four mNGS analyses were performed within two consecutive weeks. Various respiratory fungal pathogens, including Candida orthopsilosis, Candida albicans, and Aspergillus fumigatus were detected by mNGS of bronchoalveolar lavage fluid (BALF). Based on conventional pathogen identification and clinical symptoms, the patient was diagnosed with severe pneumonia caused by a fungal infection. The abundance of fungal species decreased gradually in response to antifungal and empirical therapies, and the fungal infections were effectively controlled. In summary, our results demonstrated that mNGS could effectively identify pathogens in patients with severe pneumonia. Additionally, dynamic pathogen monitoring based on mNGS could assist in the precise diagnosis of complex infections and may facilitate rapid induction of the most appropriate therapy. | ||
| 546 | |a EN | ||
| 690 | |a Metagenomic next-generation sequencing | ||
| 690 | |a Severe pneumonia | ||
| 690 | |a Dynamic detection | ||
| 690 | |a Precision diagnosis | ||
| 690 | |a Respiratory pathogens | ||
| 690 | |a Infectious and parasitic diseases | ||
| 690 | |a RC109-216 | ||
| 690 | |a Public aspects of medicine | ||
| 690 | |a RA1-1270 | ||
| 655 | 7 | |a article |2 local | |
| 786 | 0 | |n Biosafety and Health, Vol 5, Iss 3, Pp 138-143 (2023) | |
| 787 | 0 | |n http://www.sciencedirect.com/science/article/pii/S2590053623000484 | |
| 787 | 0 | |n https://doaj.org/toc/2590-0536 | |
| 856 | 4 | 1 | |u https://doaj.org/article/004ad39d3a9b43d4be85a97eb76111d6 |z Connect to this object online. |