Using Tissue Culture Technique for the Production of Cardiac Glycosides From Roots of Digitalis purpurea L Plantlets
The present study was performed using the tissue culture technique , where by roots formation was stimulated shoot tips of Digitalis purpurea L plant using auxins in this study ,shoot tips (1cm length ) were excised from sterilized seedlings of Digitalis purpurea L.Var Excelsior mixed , then they w...
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| Main Authors: | , |
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| Format: | Book |
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College of Pharmacy University of Baghdad,
2017-03-01T00:00:00Z.
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| Summary: | The present study was performed using the tissue culture technique , where by roots formation was stimulated shoot tips of Digitalis purpurea L plant using auxins in this study ,shoot tips (1cm length ) were excised from sterilized seedlings of Digitalis purpurea L.Var Excelsior mixed , then they were cultured on MS medium with the addition of one of the auxins IAA, IBA ,NAA of different concentration ( 0.0 ,0.05 ,0.1 ,0.5 and 1.0 mg / L ). After 45 days of starting the culture results showed that the treatment 0.5 mg/ L of IBA had a high significant effect on the dry weight of roots formation and containing Digitoxin and Gitoxin whose quantities as a rate of ( 2.96 gm , 30.01 and 11.05 mg/gm dry weight respectively .Also this treatment has given highest values with the other studied characteristics where the percent of the soluble sugars and starch and they are ( 2.48 % , 2.82%) respectively Abbreviation (Indole Butyric Acid )IBA , ( Indole Acetic Acid )IAA , ( Murashige and skoog medium , 1962 ) MS , (Naphthalen acetic Acetic ) NAA , ( Milligram ) Mg , ( Microgram )ug , (Litter ) L , (Gram) gm . |
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| Item Description: | 2521-3512 1683-3597 |