Phenylalanine ammonia-lyase through evolution: A bioinformatic approach

• Main Author: Shiva Hemmati (Author)
• Format: Book
• Published: Shiraz University of Medical Sciences, 2015-02-01T00:00:00Z.
• Subjects: article
• Online Access: Connect to this object online.

Summary

<p>Phenylalanine ammonia-lyase (PAL) is the first entry enzyme of the phenylpropanoid pathway that converts phenylalanine to cinnamic acid which is the precursor of various secondary metabolites. PAL is recently formulated for Phenylketonuric patients in pegylated forms. Screening a PAL with the highest affinity to the substrate is of great importance for this purpose.  PAL exists in all higher plants and some fungi and few bacteria. Ancestors of land plants have been adopted by evolving metabolic pathways. A multi-gene family encodes PAL by gene duplication events in most plants. In this study, the taxonomic distribution and phylogeny of<em> pal</em> gene found in land plants, fungi and bacteria have been analyzed. It seems that the ancestor of plants acquired a <em>pal</em> gene via horizontal gene transfer in symbioses with bacteria and fungi. Gymnosperms have kept a diverse set of <em>pal </em>genes that arose from gene duplication events. In angiosperms, after the divergence of dicotyledons from monocots,<em> pal</em> genes were duplicated many times. The close paralogues of <em>pal</em> genes in some species indicate expansion of gene families after the divergence in plant <em>pal</em> gene evolution. Interestingly, some of the plant <em>pal</em>s clustered by species a way that <em>pal</em>s within one species are more closely related to each other than to homologs in the other species which indicates this duplication event occurred more recently.</p>

Item Description

2423-3722
2423-5652
10.1111/tips.v1i1.12