Development of RT-qPCR and semi-nested RT-PCR assays for molecular diagnosis of hantavirus pulmonary syndrome.

• Main Authors: Bruno Tardelli Diniz Nunes (Author), Maria Helena Rodrigues de Mendonça (Author), Darlene de Brito Simith (Author), Adriana Freitas Moraes (Author), Carla Conceição Cardoso (Author), Ivy Tsuya Essashika Prazeres (Author), Ana Alice de Aquino (Author), Alessandra da Conceição Miranda Santos (Author), Alice Louize Nunes Queiroz (Author), Daniela Sueli Guerreiro Rodrigues (Author), Regis Bruni Andriolo (Author), Elizabeth Salbé Travassos da Rosa (Author), Livia Carício Martins (Author), Pedro Fernando da Costa Vasconcelos (Author), Daniele Barbosa de Almeida Medeiros (Author)
• Format: Book
• Published: Public Library of Science (PLoS), 2019-12-01T00:00:00Z.
• Subjects: article
• Online Access: Connect to this object online.

Hantavirus Pulmonary Syndrome is an, often fatal, emerging zoonotic disease in the Americas caused by hantaviruses (family: Hantaviridae). In Brazil, hantavirus routine diagnosis is based on serology (IgM-ELISA) while RT-PCR is often used to confirm acute infection. A Semi-nested RT-PCR and an inter...