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Early Response of Antimicrobial Resistance and Virulence Genes Expression in Classical, Hypervirulent, and Hybrid hvKp-MDR <i>Klebsiella pneumoniae</i> on Antimicrobial Stress

<i>Klebsiella pneumoniae</i> is an increasingly important hospital pathogen. Classical <i>K. pneumoniae</i> (cKp) and hypervirulent <i>K. pneumoniae</i> (hvKp) are two distinct evolutionary genetic lines. The recently ongoing evolution of <i>K. pneumoniae<...

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Main Authors:	Anastasiia D. Fursova (Author), Mikhail V. Fursov (Author), Evgenii I. Astashkin (Author), Tatiana S. Novikova (Author), Galina N. Fedyukina (Author), Angelina A. Kislichkina (Author), Irina A. Alexandrova (Author), Olga N. Ershova (Author), Ivan A. Dyatlov (Author), Nadezhda K. Fursova (Author)
Format:	Book
Published:	MDPI AG, 2021-12-01T00:00:00Z.
Subjects:	article
Online Access:	Connect to this object online.
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Summary:	<i>Klebsiella pneumoniae</i> is an increasingly important hospital pathogen. Classical <i>K. pneumoniae</i> (cKp) and hypervirulent <i>K. pneumoniae</i> (hvKp) are two distinct evolutionary genetic lines. The recently ongoing evolution of <i>K. pneumoniae</i> resulted in the generation of hybrid hvKP-MDR strains. <i>K. pneumoniae</i> distinct isolates (<i>n</i> = 70) belonged to 20 sequence types with the prevalence of ST395 (27.1%), ST23 (18.6%), ST147 (15.7%), and ST86 (7.1%), and 17 capsular types with the predominance of K2 (31.4%), K57 (18.6%), K64 (10.0%), K1 (5.7%) were isolated from patients of the Moscow neurosurgery ICU in 2014-2019. The rate of multi-drug resistant (MDR) and carbapenem-resistant phenotypes were 84.3% and 45.7%, respectively. Whole-genome sequencing of five selected strains belonging to cKp (ST395<sup>K47</sup> and ST147<sup>K64</sup>), hvKp (ST86<sup>K2</sup>), and hvKp-MDR (ST23<sup>K1</sup> and ST23<sup>K57</sup>) revealed <i>bla</i><sub>SHV</sub>, <i>bla</i><sub>TEM</sub>, <i>bla</i><sub>CTX</sub>, <i>bla</i><sub>OXA-48</sub>, and <i>bla</i><sub>NDM</sub> beta-lactamase genes; <i>acr</i>, <i>oqx</i>, <i>kpn</i>, <i>kde</i>, and <i>kex</i> efflux genes; and <i>K. pneumoniae</i> virulence genes. Selective pressure of 100 mg/L ampicillin or 10 mg/L ceftriaxone induced changes of expression levels for named genes in the strains belonging to cKp, hvKp, and hybrid hvKp-MDR. Obtained results seem to be important for epidemiologists and clinicians for enhancing knowledge about hospital pathogens.
Item Description:	10.3390/antibiotics11010007 2079-6382