Effects of Bunium persicum essential oil on the reduction of spore germination, growth, and expression of FUM1 and FUM14 genes in Fusarium verticillioides isolates
Background and Purpose: Black Cumin of Kerman (Bunium persicum) is an Iranian plant that is commonly used as an antispasmodic, carminative, and antimicrobial substance. The present study aimed to assess different components of the essence of B.persicum and its effect on antifungal activity, spore ge...
Saved in:
| Main Authors: | , , , |
|---|---|
| Format: | Book |
| Published: |
Mazandaran University of Medical Sciences,
2021-06-01T00:00:00Z.
|
| Subjects: | |
| Online Access: | Connect to this object online. |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| Summary: | Background and Purpose: Black Cumin of Kerman (Bunium persicum) is an Iranian plant that is commonly used as an antispasmodic, carminative, and antimicrobial substance. The present study aimed to assess different components of the essence of B.persicum and its effect on antifungal activity, spore germination inhibition, and expressions of FUM1 and FUM14 genes in Fusarium verticillioides strains.Materials and Methods: The essence was extracted by hydrodistillation and analyzed through gas chromatography-mass spectroscopy. A broth microdilution method was used for the determination of the minimum inhibitory concentration (MIC). In addition, the expression of FUM1 and FUM14 genes of toxigenic F. verticillioides was assessed byusing the real-time polymerase chain reaction (RT-PCR) technique.Results: Based on the findings, most of the essence consisted of γ-terpinene (15.56%),propanal, and 2-methyl-3-phenyl (14.18%). The oil showed a good antifungal activity(mean MIC value: 2556.8 μg/ml) as well as the inhibition of spore germination and mycelial growth (P<0.05). The RT-PCR demonstrated that the expression levels of FUM1 and FUM14 of B. persicum-treated F. verticillioides were 0.43 and 0.53 folds ower than the control samples, respectively.Conclusion: These findings revealed that the essential oil of B. persicum has different components responsible for the inhibition of mycelial growth and spore germination of F. verticillioides as well as reduction of expressions of FUM1 and FUM14 genes involving fumonisin production. |
|---|---|
| Item Description: | 2423-3439 2423-3420 10.18502/CMM.7.2.7033 |