Sex determination by amplification of amelogenin gene from dental pulp tissue by polymerase Chain Reaction

Introduction: Forensic odontology necessarily involves the application of dentistry along with various other branches of sciences which deals with proper handling, examination, evaluation, and presentation of dental evidences, that aids to investigate a crime and deliver justice. Sex determination i...

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Bibliographic Details
Main Authors: Ripon Md Chowdhury (Author), Abhishek Singhvi (Author), Neeta Bagul (Author), Sanya Bhatia (Author), Gurdeep Singh (Author), Surajit Goswami (Author)
Format: Book
Published: Wolters Kluwer Medknow Publications, 2018-01-01T00:00:00Z.
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042 |a dc 
100 1 0 |a Ripon Md Chowdhury  |e author 
700 1 0 |a Abhishek Singhvi  |e author 
700 1 0 |a Neeta Bagul  |e author 
700 1 0 |a Sanya Bhatia  |e author 
700 1 0 |a Gurdeep Singh  |e author 
700 1 0 |a Surajit Goswami  |e author 
245 0 0 |a Sex determination by amplification of amelogenin gene from dental pulp tissue by polymerase Chain Reaction 
260 |b Wolters Kluwer Medknow Publications,   |c 2018-01-01T00:00:00Z. 
500 |a 0970-9290 
500 |a 1998-3603 
500 |a 10.4103/ijdr.IJDR_274_17 
520 |a Introduction: Forensic odontology necessarily involves the application of dentistry along with various other branches of sciences which deals with proper handling, examination, evaluation, and presentation of dental evidences, that aids to investigate a crime and deliver justice. Sex determination is a part of forensic odontology and an essential priority when traditional identification of the deceased becomes impossible. Aim: To determine Sex by analysis of the Amelogenin gene using Polymerase Chain Reaction (PCR) method on Deoxyribose nucleic acid (DNA) isolated from dental pulp, which was exposed to various environmental conditions created artificially to mimic a forensic scenario. Materials and Method: This in-vitro study was conducted by subjecting extracted teeth to various conditions imitating a forensic scene, viz. desiccation at room temperatures, immersion in salt water, burial in soil and even exposing to extremes of temperatures. DNA was extracted from dental pulp tissue and sex determination was achieved by amplification of the amelogenin gene through AMEL gene based primers in PCR. Result: Among all the samples used in this study, DNA could be extracted from all, except from those that were subjected to a temperature of 350 °C. DNA amplification and sex determination of the samples were found to be accurate when compared to sex of the individual which was recorded initially, during collection of teeth samples. Conclusion: This study shows teeth to be a potent source of DNA even in extreme environmental conditions, barring high temperatures and determination of sex by PCR amplification of AMEL markers to be quite reliable. 
546 |a EN 
690 |a Amelogenin 
690 |a dental pulp 
690 |a DNA 
690 |a environmental conditions 
690 |a sex determination 
690 |a Dentistry 
690 |a RK1-715 
655 7 |a article  |2 local 
786 0 |n Indian Journal of Dental Research, Vol 29, Iss 4, Pp 470-476 (2018) 
787 0 |n http://www.ijdr.in/article.asp?issn=0970-9290;year=2018;volume=29;issue=4;spage=470;epage=476;aulast=Chowdhury 
787 0 |n https://doaj.org/toc/0970-9290 
787 0 |n https://doaj.org/toc/1998-3603 
856 4 1 |u https://doaj.org/article/0475f0bc02c74faaab6ebb0c9971a17f  |z Connect to this object online.