Antibacterial activities of the extracts, fractions and compounds from <it>Dioscorea bulbifera</it>
<p>Abstract</p> <p>Background</p> <p><it>Dioscorea bulbifera</it> is an African medicinal plant used to treat microbial infections. In the present study, the methanol extract, fractions (DBB1 and DBB2) and six compounds isolated from the bulbils of <it>...
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| Main Authors: | , , , , , , |
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| Format: | Book |
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BMC,
2012-11-01T00:00:00Z.
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| Summary: | <p>Abstract</p> <p>Background</p> <p><it>Dioscorea bulbifera</it> is an African medicinal plant used to treat microbial infections. In the present study, the methanol extract, fractions (DBB1 and DBB2) and six compounds isolated from the bulbils of <it>D</it>. <it>bulbifera</it>, namely bafoudiosbulbins A (<b>1</b>), B (<b>2</b>), C (<b>3</b>), F (<b>4</b>), G (<b>5</b>) and 2,7-dihydroxy-4-methoxyphenanthrene (<b>6</b>), were tested for their antimicrobial activities against Mycobacteria and Gram-negative bacteria involving multidrug resistant (MDR) phenotypes expressing active efflux pumps.</p> <p>Methods</p> <p>The microplate alamar blue assay (MABA) and the broth microdilution methods were used to determine the minimal inhibitory concentration (MIC) and minimal bactericidal concentration (MBC) of the above samples.</p> <p>Results</p> <p>The results of the MIC determinations indicated that when tested alone, the crude extract, fractions DBB1 and DBB2 as well as compounds <b>2</b> to <b>5</b> were able to prevent the growth of all the fifteen studied microorganisms, within the concentration range of 8 to 256 μg/mL. The lowest MIC value for the methanol extract and fractions (16 μg/mL) was obtained with DBB1 and DBB2 on <it>E</it>, <it>coli</it> AG100A and DBB2 on <it>Mycobacterium tuberculosis</it> MTCS2. The lowest value for individual compounds (8 μg/mL) was recorded with compound <b>3</b> on <it>M</it>. <it>smegmatis</it> and <it>M</it>. <it>tuberculosis</it> ATCC and MTCS2 strains respectively. The activity of the samples on many MDR bacteria such as <it>Enterobacter aerogenes</it> EA289, CM64, <it>Klebsiella pneumoniae</it> KP63 and <it>Pseudomonas aeruginosa</it> PA124 was better than that of chloramphenicol. When tested in the presence of the efflux pump inhibitor against MDR Gram-negative bacteria, the activity of most of the samples increased. MBC values not greater than 512 μg/mL were recorded on all studied microorganisms with fraction DBB2 and compounds <b>2</b> to <b>5</b>.</p> <p>Conclusions</p> <p>The overall results of the present investigation provided evidence that the crude extract <it>D</it>. <it>bulbifera</it> as well as some of the compounds and mostly compounds <b>3</b> could be considered as potential antimicrobial drugs to fight against MDR bacteria.</p> |
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| Item Description: | 10.1186/1472-6882-12-228 1472-6882 |