Evaluation of the Hemocompatibility and Anticancer Potential of Poly(<i>ε</i>-Caprolactone) and Poly(3-Hydroxybutyrate) Microcarriers with Encapsulated Chrysin
In this work, novel chrysin-loaded poly(<i>ε</i>-caprolactone) and poly(3-hydroxybutyrate) microcarriers were synthesized according to a modified oil-in-water single emulsion/solvent evaporation method, utilizing poly(vinyl alcohol) surfactant as stabilizer and dispersing agent for the e...
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| Main Authors: | , , , , , , , |
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| Format: | Book |
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MDPI AG,
2021-01-01T00:00:00Z.
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| Summary: | In this work, novel chrysin-loaded poly(<i>ε</i>-caprolactone) and poly(3-hydroxybutyrate) microcarriers were synthesized according to a modified oil-in-water single emulsion/solvent evaporation method, utilizing poly(vinyl alcohol) surfactant as stabilizer and dispersing agent for the emulsification, and were evaluated for their physico-chemical and morphological properties, loading capacity and entrapment efficiency and in vitro release of their load. The findings suggest that the novel micro-formulations possess a spherical and relatively wrinkled structure with sizes ranging between 2.4 and 24.7 µm and a highly negative surface charge with <i>z</i>-potential values between (−18.1)-(−14.1) mV. The entrapment efficiency of chrysin in the poly(<i>ε</i>-caprolactone) and poly(3-hydroxybutyrate) microcarriers was estimated to be 58.10% and 43.63%, whereas the loading capacity was found to be 3.79% and 15.85%, respectively. The average release percentage of chrysin was estimated to be 23.10% and 18.01%, respectively. The novel micromaterials were further biologically evaluated for their hemolytic activity through hemocompatibility studies over a range of hematological parameters and cytoxicity against the epithelial human breast cancer cell line MDA-MB 231. The poly(<i>ε</i>-caprolactone) and poly(3-hydroxybutyrate) microcarriers reached an IC<sub>50</sub> value with an encapsulated chrysin content of 149.19 µM and 312.18 µM, respectively, and showed sufficient blood compatibility displaying significantly low (up to 2%) hemolytic percentages at concentrations between 5 and 500 µg·mL<sup>−1</sup>. |
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| Item Description: | 10.3390/pharmaceutics13010109 1999-4923 |