The influence of dehydroepiandrosterone on effector functions of neutrophils

• Main Authors: Verônica Soares Brauer (Author), Fabiana Albani Zambuzi (Author), Milena Sobral Espíndola (Author), Marinaldo Pacífico Cavalcanti Neto (Author), Morgana Kelly Borges Prado (Author), Priscilla Mariane Cardoso (Author), Luana Silva Soares (Author), Leonardo Judson Galvao-Lima (Author), Andréia Machado Leopoldino (Author), Cristina Ribeiro de Barros Cardoso (Author), Fabiani Gai Frantz (Author)
• Format: Book
• Published: Universidade de São Paulo, 2021-11-01T00:00:00Z.
• Subjects: article
• Online Access: Connect to this object online.

Summary

Dehydroepiandrosterone (DHEA) is a steroid hormone secreted by the adrenal glands, gonads and brain. It is a precursor to sex hormones and also is known to have immune modulatory activity. However, little is known about the relationship between DHEA and neutrophils and thus our study evaluates the influence of DHEA in the effector functions of neutrophils. Human neutrophils were treated in vitro with DHEA and further infected with Salmonella enterica serovar Typhimurium. The treatment of neutrophils with 0.01 μM of DHEA increased the phagocytosis of Salmonella independent of TLR4 as the treatment did not modulate the TLR4 expression. Additionally, DHEA caused a decrease in ROS (reactive oxygen species) production and did not influence the formation of the neutrophil extracellular trap (NET). Steroid treated neutrophils, infected or stimulated with LPS (lipopolysaccharide), showed reduced production of IL-8, compared to untreated cells. Also, the protein levels of p-NFκB were decreased in neutrophils treated with DHEA, and this reduction could explain the reduced levels of IL-8. These results led us to conclude that the steroid hormone DHEA has important modulatory functions in neutrophils.

Item Description

2175-9790
10.1590/s2175-97902020000419139