Successful and failed mini-implants: microbiological evaluation and quantification of bacterial endotoxin

• Main Authors: Marcela Cristina Damião ANDRUCIOLI (Author), Mírian Aiko Nakane MATSUMOTO (Author), Maria Conceição Pereira SARAIVA (Author), Magda FERES (Author), Luciene Cristina de FIGUEIREDO (Author), Carlos Artério SORGI (Author), Lucia Helena FACCIOLI (Author), Raquel Assed Bezerra da SILVA (Author), Lea Assed Bezerra da SILVA (Author), Paulo NELSON-FILHO (Author)
• Format: Book
• Published: University of São Paulo, 2018-07-01T00:00:00Z.
• Subjects: article
• Online Access: Connect to this object online.

Summary

Abstract Objectives Using two groups of mini-implants (successful and failed) the objectives of this in vivo study were: to evaluate the microbial contamination by the checkerboard DNA-DNA hybridization technique and to quantify the bacterial endotoxin by the limulus amebocyte lysate assay. Material and Methods The 15 successful and 10 failed mini-implants (1.6 mm diameter × 7.0 or 9.0 mm long), placed in the maxilla and/or mandible, were obtained from 15 patients undergoing orthodontic treatment. Data were analyzed statistically by the Wilcoxon rank-sum test using the SAS software (a=0.05). Results All 40 microbial species were detected in both groups of mini-implants, with different frequencies. No differences were observed between the groups with respect to microbial complexes (blue, purple, yellow, green, orange, red and other species) and endotoxin quantification (p>0.05). Conclusion Neither microbial contamination nor endotoxin quantification was determinant for the early loss of stability of the mini-implants.

Item Description

1678-7765
10.1590/1678-7757-2017-0631