O-(β-hydroxyethyl)rutosides determination by micellar flow injection (FI)-spectrofluorimetry
A simple, eco-friendly, sensitive and economic flow injection spectrofluorimetric method was developed for the determination of O-(β-hydroxyethyl)rutosides. The procedure was based on the use of an anionic surfactant such as sodium dodecyl sulfate to provide an appreciable O-(β-hydroxyethyl)rutosi...
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| Main Authors: | , |
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| Format: | Book |
| Published: |
Elsevier,
2014-12-01T00:00:00Z.
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| Summary: | A simple, eco-friendly, sensitive and economic flow injection spectrofluorimetric method was developed for the determination of O-(β-hydroxyethyl)rutosides. The procedure was based on the use of an anionic surfactant such as sodium dodecyl sulfate to provide an appreciable O-(β-hydroxyethyl)rutosides fluorescence enhancement, increasing considerably the sensitivity of detection. All the variables affecting the fluorescence intensity were studied and optimized. The flow rate was 5 mL/min with detection at 450 nm (after excitation at 346 nm). A linear correlation between drug amount and peak area was established for O-(β-hydroxyethyl)rutosides in the range of 0.01â200 µg/mL with a detection limit of 0.001 µg/mL (s/n=3). Validation processes were performed by recovering studies with satisfactory results. The new methodology can be employed for the routine analysis of O-(β-hydroxyethyl)rutosides in bulks as well as in commercial formulations. Keywords: O-(β-hydroxyethyl)rutosides, Micellar enhancement, Flow injection, Spectrofluorimetry, Pharmaceuticals |
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| Item Description: | 2095-1779 10.1016/j.jpha.2014.07.002 |