Long-Term Tumor-Targeting Effect of <i>E. coli</i> as a Drug Delivery System

• Main Authors: Gun Gyun Kim (Author), Hongje Lee (Author), Dan Bi Jeong (Author), Sang Wook Kim (Author), Jae-Seon So (Author)
• Format: Book
• Published: MDPI AG, 2024-03-01T00:00:00Z.
• Subjects: article
• Online Access: Connect to this object online.

Summary

To overcome the limitations of current nano/micro-scale drug delivery systems, an <i>Escherichia coli</i> (<i>E. coli</i>)-based drug delivery system could be a potential alternative, and an effective tumor-targeting delivery system can be developed by attempting to perform chemical binding to the primary amine group of a cell membrane protein. In addition, positron emission tomography (PET) is a representative non-invasive imaging technology and is actively used in the field of drug delivery along with radioisotopes capable of long-term tracking, such as zirconium-89 (⁸⁹Zr). The membrane proteins were labeled with ⁸⁹Zr using chelate (DFO), and not only was the long-term biodistribution in tumors and major organs evaluated in the body, but the labeling stability of ⁸⁹Zr conjugated to the membrane proteins was also evaluated through continuous tracking. <i>E. coli</i> accumulated at high levels in the tumor within 5 min (initial time) after tail intravenous injection, and when observed after 6 days, ⁸⁹Zr-DFO on the surface of <i>E. coli</i> was found to be stable for a long period of time in the body. In this study, we demonstrated the long-term biodistribution and tumor-targeting effect of an <i>E. coli</i>-based drug delivery system and verified the in vivo stability of radioisotopes labeled on the surface of <i>E. coli</i>.

Item Description

10.3390/ph17040421
1424-8247