Validation and Application of an HPLC-UV Method for Routine Therapeutic Drug Monitoring of Cefiderocol
Cefiderocol is a new siderophore cephalosporin approved for the treatment of multidrug resistant bacteria including activity against carbapenem-resistant Enterobacterales and <i>Pseudomonas aeruginosa</i>. As cephalosporins are known for their high pharmacokinetic variability in critical...
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| Main Authors: | , , , , , , |
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| Format: | Book |
| Published: |
MDPI AG,
2021-02-01T00:00:00Z.
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| Summary: | Cefiderocol is a new siderophore cephalosporin approved for the treatment of multidrug resistant bacteria including activity against carbapenem-resistant Enterobacterales and <i>Pseudomonas aeruginosa</i>. As cephalosporins are known for their high pharmacokinetic variability in critically ill patients, cefiderocol therapeutic drug monitoring might become a valuable tool. Therefore, we aimed to develop and validate a simple, rapid, cost-effective high performance liquid chromatography (HPLC) method for the quantification of cefiderocol in serum. Samples were treated for protein precipitation followed by chromatographic separation on a reverse phase column (HPLC C-18) with gradient elution of the mobile phase. Cefiderocol was detected via UV absorption and quantification was performed with the internal standard (metronidazole) method. The calibration range showed linearity from 4 to 160 mg/L. The intra and interday precision was less than 10% with a recovery rate of 81%. The method was successfully used for the analysis of subsequent serum samples of critically ill patients and showed good performance in monitoring serum levels and optimizing antibiotic therapy. |
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| Item Description: | 10.3390/antibiotics10030242 2079-6382 |