Efficacy of Thai Plant Extracts for Antibacterial and Anti-Biofilm Activities against Pathogenic Bacteria
The emergence of drug-resistant bacteria has impacted the outcome of current therapeutics as a threat to global healthcare; novel medicines are urgently needed. Thirteen medicinal plants were collected in Northeastern Thailand, and their crude ethanolic extracts were evaluated for antibacterial acti...
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| Main Authors: | , |
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| Format: | Book |
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MDPI AG,
2021-11-01T00:00:00Z.
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| Summary: | The emergence of drug-resistant bacteria has impacted the outcome of current therapeutics as a threat to global healthcare; novel medicines are urgently needed. Thirteen medicinal plants were collected in Northeastern Thailand, and their crude ethanolic extracts were evaluated for antibacterial activities against <i>Staphylococcus aureus</i> ATCC25923 and <i>Escherichia coli</i> ATCC25922 using the broth micro-dilution method. <i>Piper betle</i> leaf ethanolic extract showed optimal activity against both representative bacterial strains. Activity was also observed against clinical isolates of methicillin-resistant <i>S. aureus</i> (MRSA) and <i>E. coli</i>, with minimal inhibitory concentration (MIC) ranging from 0.31 mg/mL to 2.5 mg/mL and minimal bactericidal concentration (MBC) ranging from 0.62 mg/mL to 2.5 mg/mL. A time-kill study revealed that the extract activity was time- and dose-dependent, and also bactericidal on the tested bacteria. <i>P. betle</i> extract inhibited biofilm formation and promoted biofilm eradication in both <i>S. aureus</i> and <i>E. coli.</i> 4-Allyl-1,2-diacetoxybenzene and eugenol were identified as the most abundant compounds in the extract and may play major roles in the anti-bacterial and anti-biofilm activity. Results suggest that ethanolic <i>P. betle</i> leaf extract shows promise as an alternative method for the prevention of bacterial diseases. |
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| Item Description: | 10.3390/antibiotics10121470 2079-6382 |