Preclinical study of experimental burns treated with photobiomodulation and Human Amniotic Membrane, both isolated and associated

• Main Authors: Fernanda Cláudia Miranda Amorim (Author), Emilia Ângela Loschiavo Arisawa (Author), Luciana Barros Sant'anna (Author), Ana Beatriz Mendes Rodrigues (Author), Davidson Ribeiro Costa (Author)
• Format: Book
• Published: Universidade de São Paulo, 2023-03-01T00:00:00Z.
• Subjects: article
• Online Access: Connect to this object online.
• Notes: 1518-8345; 10.1590/1518-8345.5552.3726
• Keywords: Burns; Amnion; Low-Level Light Therapy; Wound Healing; Skin; Rats.; Nursing; RT1-120
• Source: Revista Latino-Americana de Enfermagem, Vol 31 (2023)

Abstract Objective: to evaluate the effect of photobiomodulation with low-level 660 nm laser alone or associated with Human Amniotic Membrane in the repair of partial-thickness burns in rats. Method: an experimental study conducted with 48 male Wistar rats, randomized into four groups: Control, Human Amniotic Membrane, Low-Level Laser Therapy, and Low-Level Laser Therapy associated with Human Amniotic Membrane. The histopathological characteristics of the skin samples were analyzed 7 and 14 days after the burn. The data obtained were submitted to the Kolmogorov-Smirnov and Mann-Whitney tests. Results: the histological analysis of the burn injuries showed a decrease in inflammation (p<0.0001) and an increase in proliferation of fibroblasts (p<0.0001) mainly at 7 days in all treatments related to the control group. At 14 days, the greater effectiveness in accelerating the healing process was significant (p<0.0001) in the Low-Level Laser Therapy group associated with the Human Amniotic Membrane. Conclusion: the association of photobiomodulation therapies with the Human Amniotic Membrane allowed verifying a reduction in the healing process time of the experimental lesions, stimulating its proposal as a treatment protocol in partial-thickness burns.