Antiproliferative activity of extract from in vitro callus cultures of Astragalus vesicarius ssp. carniolicus (A. Kern.) Chater

• Main Authors: Pavlinka Popova (Author), Yancho Zarev (Author), Rositsa Mihaylova (Author), Georgi Momekov (Author), Iliana Ionkova (Author)
• Format: Book
• Published: Pensoft Publishers, 2021-02-01T00:00:00Z.
• Subjects: article
• Online Access: Connect to this object online.

Summary

Five isoflavonoids, i.e. 5-hydroxy-7-methoxy-2', 5'-dihydroxyisoflavone (AV4), 5, 7-dihydroxy-4'-methoxyisoflavone (AV6), 7-methoxy-5-hydroxy-4'-methoxy-2'-hydroxyisoflavone (AV7), 8-pregnyl genistein (AV9), 5,7-dihydroxy-8-pregnyl-4'-methoxy-2'-hydroxyisoflavone (AV10) and one coumarochromone - sophorophenolone (AV8) were isolated from EtOAc of in vitro callus cultures of Astragalus vesicarius ssp. carniolicus, after enzymatic hydrolysis with β-glucosidase. Their structures were tentatively elucidated by spectroscopic mean (1H NMR and HR-ESI-MS spectra). Antiproliferative activity of EtOAc extract and isolated aglycones against chemosensitive human promyelocyte cell line HL-60 and its multidrug-resistant variant HL-60/Dox was assessed in vitro. Despite the strong activity of EtOAc (IC50 8.8 µg/mL (HL-6, 72 h) to 11.8 µg/mL (HL-60/Dox, 72 h)), prenylated compound AV9 showed also antiproliferative activity - 36.1 µg/mL (HL-60 and HL-60/Dox, 72 h).

Item Description

10.3897/pharmacia.68.e63421
2603-557X