The roles of DNA methylation and hydroxymethylation at short interspersed nuclear elements in the hypothalamic arcuate nucleus during puberty
Puberty is the gateway to adult reproductive competence, encompassing a suite of complex, integrative, and coordinated changes in neuroendocrine functions. However, the regulatory mechanisms of transcriptional reprogramming in the arcuate nucleus (ARC) during onset of puberty are still not fully und...
Saved in:
| Main Authors: | , , , , , , |
|---|---|
| Format: | Book |
| Published: |
Elsevier,
2021-12-01T00:00:00Z.
|
| Subjects: | |
| Online Access: | Connect to this object online. |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
MARC
| LEADER | 00000 am a22000003u 4500 | ||
|---|---|---|---|
| 001 | doaj_0d0122c46e9843cda2ac9e09877f5c00 | ||
| 042 | |a dc | ||
| 100 | 1 | 0 | |a Yihang Shen |e author |
| 700 | 1 | 0 | |a Hongchao Zhao |e author |
| 700 | 1 | 0 | |a Lei Zhang |e author |
| 700 | 1 | 0 | |a Yanping Hu |e author |
| 700 | 1 | 0 | |a Li Cai |e author |
| 700 | 1 | 0 | |a Jun Li |e author |
| 700 | 1 | 0 | |a Shasha Zhou |e author |
| 245 | 0 | 0 | |a The roles of DNA methylation and hydroxymethylation at short interspersed nuclear elements in the hypothalamic arcuate nucleus during puberty |
| 260 | |b Elsevier, |c 2021-12-01T00:00:00Z. | ||
| 500 | |a 2162-2531 | ||
| 500 | |a 10.1016/j.omtn.2021.07.006 | ||
| 520 | |a Puberty is the gateway to adult reproductive competence, encompassing a suite of complex, integrative, and coordinated changes in neuroendocrine functions. However, the regulatory mechanisms of transcriptional reprogramming in the arcuate nucleus (ARC) during onset of puberty are still not fully understood. To understand the role of epigenetics in regulating gene expression, mouse hypothalamic ARCs were isolated at 4 and 8 weeks, and the transcriptome, DNA hydroxymethylation, DNA methylation, and chromatin accessibility were assessed via RNA sequencing (RNA-seq), reduced representation bisulfite sequencing (RRBS-seq), reduced representation hydroxymethylation profiling (RRHP)-seq, and assay for transposase-accessible chromatin (ATAC-seq), respectively. The overall DNA hydroxymethylation and DNA methylation changes in retroelements (REs) were associated with gene expression modeling for puberty in the ARC. We focused on analyzing DNA hydroxymethylation and DNA methylation at two short interspersed nuclear elements (SINEs) located on the promoter of the 5-hydroxytryptamine receptor 6 (Htr6) gene and the enhancer of the KISS-1 metastasis suppressor (Kiss1) gene and investigated their regulatory roles in gene expression. Our data uncovered a novel epigenetic mechanism by which SINEs regulate gene expression during puberty. | ||
| 546 | |a EN | ||
| 690 | |a arcuate nucleus | ||
| 690 | |a DNA methylation | ||
| 690 | |a DNA hydroxymethylation | ||
| 690 | |a puberty | ||
| 690 | |a retroelement | ||
| 690 | |a short interspersed nuclear elements | ||
| 690 | |a Therapeutics. Pharmacology | ||
| 690 | |a RM1-950 | ||
| 655 | 7 | |a article |2 local | |
| 786 | 0 | |n Molecular Therapy: Nucleic Acids, Vol 26, Iss , Pp 242-252 (2021) | |
| 787 | 0 | |n http://www.sciencedirect.com/science/article/pii/S2162253121001748 | |
| 787 | 0 | |n https://doaj.org/toc/2162-2531 | |
| 856 | 4 | 1 | |u https://doaj.org/article/0d0122c46e9843cda2ac9e09877f5c00 |z Connect to this object online. |