Development of new HPLC method for identification of metabolic degradation of N-pyrrolylhydrazide hydrazones with determined MAO- B activity in cellular cultures
In this research, a new rapid PR- HPLC method was developed for the determination of metabolites in isolated rat hapatocytes. The chromatographic parameters, including the stationary and mobile phases, outlet pressure, temperature and flow rate, were optimized. The method identified two initial from...
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Pensoft Publishers,
2022-01-01T00:00:00Z.
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| 001 | doaj_0d1088daa9464a8a8934ae3e7de7d2e3 | ||
| 042 | |a dc | ||
| 100 | 1 | 0 | |a Alexandrina Mateeva |e author |
| 700 | 1 | 0 | |a Lily Peikova |e author |
| 700 | 1 | 0 | |a Magdalena Kondeva-Burdina |e author |
| 700 | 1 | 0 | |a Maya Georgieva |e author |
| 245 | 0 | 0 | |a Development of new HPLC method for identification of metabolic degradation of N-pyrrolylhydrazide hydrazones with determined MAO- B activity in cellular cultures |
| 260 | |b Pensoft Publishers, |c 2022-01-01T00:00:00Z. | ||
| 500 | |a 10.3897/pharmacia.69.e78417 | ||
| 500 | |a 2603-557X | ||
| 520 | |a In this research, a new rapid PR- HPLC method was developed for the determination of metabolites in isolated rat hapatocytes. The chromatographic parameters, including the stationary and mobile phases, outlet pressure, temperature and flow rate, were optimized. The method identified two initial from the synthesis molecules in higher concentration and one new unidentified structure as products of the hepatocytic processing of the evaluated analyte. The results identified as first step of metabolism the hydrolysis of the hydrazone group. Further investigations should be aimed into determining the next metabolic transformations, predicted by the in silico application of the web server SMARTCyp. | ||
| 546 | |a EN | ||
| 690 | |a Pharmacy and materia medica | ||
| 690 | |a RS1-441 | ||
| 655 | 7 | |a article |2 local | |
| 786 | 0 | |n Pharmacia, Vol 69, Iss 1, Pp 15-20 (2022) | |
| 787 | 0 | |n https://pharmacia.pensoft.net/article/78417/download/pdf/ | |
| 787 | 0 | |n https://pharmacia.pensoft.net/article/78417/download/xml/ | |
| 787 | 0 | |n https://pharmacia.pensoft.net/article/78417/ | |
| 787 | 0 | |n https://doaj.org/toc/2603-557X | |
| 856 | 4 | 1 | |u https://doaj.org/article/0d1088daa9464a8a8934ae3e7de7d2e3 |z Connect to this object online. |