The Rhodanese PspE Converts Thiosulfate to Cellular Sulfane Sulfur in <i>Escherichia coli</i>
Hydrogen sulfide (H<sub>2</sub>S) and its oxidation product zero-valent sulfur (S<sup>0</sup>) play important roles in animals, plants, and bacteria. Inside cells, S<sup>0</sup> exists in various forms, including polysulfide and persulfide, which are collectively...
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| Main Authors: | , , , , , , |
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| Format: | Book |
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MDPI AG,
2023-05-01T00:00:00Z.
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| Summary: | Hydrogen sulfide (H<sub>2</sub>S) and its oxidation product zero-valent sulfur (S<sup>0</sup>) play important roles in animals, plants, and bacteria. Inside cells, S<sup>0</sup> exists in various forms, including polysulfide and persulfide, which are collectively referred to as sulfane sulfur. Due to the known health benefits, the donors of H<sub>2</sub>S and sulfane sulfur have been developed and tested. Among them, thiosulfate is a known H<sub>2</sub>S and sulfane sulfur donor. We have previously reported that thiosulfate is an effective sulfane sulfur donor in <i>Escherichia coli</i>; however, it is unclear how it converts thiosulfate to cellular sulfane sulfur. In this study, we showed that one of the various rhodaneses, PspE, in <i>E. coli</i> was responsible for the conversion. After the thiosulfate addition, the ΔpspE mutant did not increase cellular sulfane sulfur, but the wild type and the complemented strain ΔpspE::pspE increased cellular sulfane sulfur from about 92 μM to 220 μM and 355 μM, respectively. LC-MS analysis revealed a significant increase in glutathione persulfide (GSSH) in the wild type and the ΔpspE::pspE strain. The kinetic analysis supported that PspE was the most effective rhodanese in <i>E. coli</i> in converting thiosulfate to glutathione persulfide. The increased cellular sulfane sulfur alleviated the toxicity of hydrogen peroxide during <i>E. coli</i> growth. Although cellular thiols might reduce the increased cellular sulfane sulfur to H<sub>2</sub>S, increased H<sub>2</sub>S was not detected in the wild type. The finding that rhodanese is required to convert thiosulfate to cellular sulfane sulfur in <i>E. coli</i> may guide the use of thiosulfate as the donor of H<sub>2</sub>S and sulfane sulfur in human and animal tests. |
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| Item Description: | 10.3390/antiox12051127 2076-3921 |