Identification of multi-drug resistant <it>Pseudomonas aeruginosa </it>clinical isolates that are highly disruptive to the intestinal epithelial barrier
<p>Abstract</p> <p>Background</p> <p>Multi-drug resistant <it>Pseudomonas aeruginosa </it>nosocomial infections are increasingly recognized worldwide. In this study, we focused on the virulence of multi-drug resistant clinical strains <it>P. aeruginosa...
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| Main Authors: | , , , , , , , |
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| Format: | Book |
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BMC,
2006-06-01T00:00:00Z.
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| Summary: | <p>Abstract</p> <p>Background</p> <p>Multi-drug resistant <it>Pseudomonas aeruginosa </it>nosocomial infections are increasingly recognized worldwide. In this study, we focused on the virulence of multi-drug resistant clinical strains <it>P. aeruginosa </it>against the intestinal epithelial barrier, since <it>P. aeruginosa </it>can cause lethal sepsis from within the intestinal tract of critically ill and immuno-compromised patients via mechanisms involving disruption of epithelial barrier function.</p> <p>Methods</p> <p>We screened consecutively isolated multi-drug resistant <it>P. aeruginosa </it>clinical strains for their ability to disrupt the integrity of human cultured intestinal epithelial cells (Caco-2) and correlated these finding to related virulence phenotypes such as adhesiveness, motility, biofilm formation, and cytotoxicity.</p> <p>Results</p> <p>Results demonstrated that the majority of the multi-drug resistant <it>P. aeruginosa </it>clinical strains were attenuated in their ability to disrupt the barrier function of cultured intestinal epithelial cells. Three distinct genotypes were found that displayed an extreme epithelial barrier-disrupting phenotype. These strains were characterized and found to harbor the <it>exoU </it>gene and to display high swimming motility and adhesiveness.</p> <p>Conclusion</p> <p>These data suggest that detailed phenotypic analysis of the behavior of multi-drug resistant <it>P. aeruginosa </it>against the intestinal epithelium has the potential to identify strains most likely to place patients at risk for lethal gut-derived sepsis. Surveillance of colonizing strains of <it>P. aeruginosa </it>in critically ill patients beyond antibiotic sensitivity is warranted.</p> |
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| Item Description: | 10.1186/1476-0711-5-14 1476-0711 |