Development and validation of analytical method for the estimation of nateglinide in rabbit plasma

• Main Authors: Nihar Ranjan Pani (Author), Lilakant Nath (Author), Akhilesh Vikram Singh (Author), Santosh Kumar Mahapatra (Author)
• Format: Book
• Published: Elsevier, 2012-12-01T00:00:00Z.
• Subjects: article
• Online Access: Connect to this object online.

Summary

Nateglinide has been widely used in the treatment of type-2 diabetics as an insulin secretogoga. A reliable, rapid, simple and sensitive reversed-phase high performance liquid chromatography (RP-HPLC) method was developed and validated for determination of nateglinide in rabbit plasma. The method was developed on Hypersil BDSC-18 column (250 mmÃ4.6 mm, 5 mm) using a mobile phase of 10 mM phosphate buffer (pH 2.5) and acetonitrile (35:65, v/v). The elute was monitored with the UVâvis detector at 210 nm with a flow rate of 1 mL/min. Calibration curve was linear over the concentration range of 25â2000 ng/mL. The retention times of nateglinide and internal standard (gliclazide) were 9.608 min and 11.821 min respectively. The developed RP-HPLC method can be successfully applied to the quantitative pharmacokinetic parameters determination of nateglinide in rabbit model. Keywords: HPLC, Nateglinide, Rabbit plasma, Pharmacokinetics

Item Description

2095-1779
10.1016/j.jpha.2012.05.002