Gas Chromatography/Mass Spectrometry characterization and antinociceptive effects of the ethanolic extract of the leaves from Clusia minor L.

• Váldodahkkit: Raisa Mangas (Dahkki), Gledys Reynaldo (Dahkki), Maria T. Dalla Vecchia (Dahkki), Kendely Aver (Dahkki), Leonardo G. Piovesan (Dahkki), Adonis Bello (Dahkki), Idania Rodeiro (Dahkki), Angela Malheiros (Dahkki), Marcia M. de Souza (Dahkki), Roberto Menéndez (Dahkki)
• Materiálatiipa: Girji
• Almmustuhtton: GarVal Editorial Ltda., 2019-01-01T00:00:00Z.
• Fáttát: article
• Liŋkkat: Connect to this object online.
• Notes: 0719-4250
• Keywords: acute mechanical hypernociception; acute nociceptive models; antinociceptive effect; Clusia minor L.; GC/MS; triterpenoids; Therapeutics. Pharmacology; RM1-950; Pharmacy and materia medica; RS1-441
• Source: Journal of Pharmacy & Pharmacognosy Research, Vol 7, Iss 1, Pp 21-30 (2019)

Context: The search of new substances with analgesic properties has grown in the last years. Brazil and Cuba have a big biodiversity allowing the study of several plants with potential pharmacological activities. Aims: To evaluate the chemical composition and potential antinociceptive effect of the ethanolic extract from Clusia minor L. leaves (Clusiaceae) in mice. Methods: Phytochemical characterization was performed by Gas Chromatography/Mass Spectrometry. Antinociceptive effect was evaluated using acetic acid, formalin, hot plate, and capsaicin models. Mechanical hypernociception was induced by intraplantar carrageenan, tumor necrosis factor α (TNFα) and prostagladin E2 (PGE2) and responses were measured after 3 h of injection. Results: Mass Spectrometry analysis allowed the identification of 16 compounds. Fatty acid derivatives, steroids, triterpenoids, and vitamin E were the main findings. The most abundant sterol was β-sitosterol (14.04%); followed by the triterpenes α-amyrin (11.94%), and β-amyrin (7.82%). Vitamin E represented the 8.44% of the total identified compounds. The evaluation of the acetic acid-induced nociception model showed that the extract was effective in reducing pain in a dose-dependent manner. This resulted in a maximal inhibition of 53 ± 4%. The extract was also effective in other pain models. Additionally, the extract presented a considerable inhibition of paw mechanical hypernociception. Conclusions: The data suggest that the antinociceptive effect of Clusia minor occurs by interaction of various mechanisms; which probably take places via central and peripheral pathway. Therefore, modulating the inflammatory and neurogenic pain.