Binding of 99mTc to human serum albumin
The specificity of 99m Tc-MDP binding to HSA was examined , the results showed that the non specific binding was 21-23% of the total binding . The range of optimum pH for the binding was 7.4-7.6 . The most suitable amounts for HSA to give maximum binding was (70mg)per incubation medium , binding see...
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| Format: | Book |
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College of Pharmacy / Mustansiriyah University,
2004-06-01T00:00:00Z.
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| Summary: | The specificity of 99m Tc-MDP binding to HSA was examined , the results showed that the non specific binding was 21-23% of the total binding . The range of optimum pH for the binding was 7.4-7.6 . The most suitable amounts for HSA to give maximum binding was (70mg)per incubation medium , binding seemed to be affected by 99m Tc- MDP concentration and also mono and divalent metal chloride salts. The association constants (ka) and maximum binding capacity (Bmax) of 99m Tc-MDP binding to its binding sites of HSA was determined from scatchard and double reciprocal plots , the results revealed that (ka) and (Bmax) values were temperature dependant. |
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| Item Description: | 10.32947/ajps.v1i1.449 1815-0993 2959-183X |