XH-14, a novel danshen methoxybenzo[b]furan derivative, exhibits anti-inflammatory properties in lipopolysaccharide-treated RAW 264.7 cells
<p>Abstract</p> <p>Background</p> <p>XH-14 isolated from <it>Salvia miltiorrhiza</it> is a bioactive component and adenosine antagonist. In the present study, we evaluated anti-inflammatory properties of XH-14 in murine macrophages.</p> <p>Method...
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| Format: | Book |
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BMC,
2013-01-01T00:00:00Z.
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| Summary: | <p>Abstract</p> <p>Background</p> <p>XH-14 isolated from <it>Salvia miltiorrhiza</it> is a bioactive component and adenosine antagonist. In the present study, we evaluated anti-inflammatory properties of XH-14 in murine macrophages.</p> <p>Methods</p> <p>RAW 264.7 murine macrophage cell line was cultured with various concentrations of XH-14 in the absence or presence of lipopolysaccharide (LPS). LPS-induced release and mRNA expression of inflammatory mediators were examined by ELISA and real-time PCR. The modification of signal pathways involved in inflammatory reactions was determined by Western blotting analysis.</p> <p>Results</p> <p>XH-14 suppressed the generation of nitric oxide (NO) and prostaglandin E<sub>2</sub>, and the expression of inducible NO synthase and cyclooxygenase-2 induced by LPS. Similarly, XH-14 inhibited the release of pro-inflammatory cytokines induced by LPS in RAW 264.7 cells. The underlying mechanism of XH-14 on anti-inflammatory action was correlated with down-regulation of mitogen-activated protein kinase and activator protein-1 activation.</p> <p>Conclusions</p> <p>XH-14 inhibits the production of several inflammatory mediators and so might be useful for the treatment of various inflammatory diseases.</p> |
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| Item Description: | 10.1186/1476-9255-10-1 1476-9255 |