Suppression of lung inflammation by the ethanol extract of Chung-Sang and the possible role of Nrf2

• Κύριοι συγγραφείς: Jin-Woo Han (Συγγραφέας), Kyun Ha Kim (Συγγραφέας), Min-Jung Kwun (Συγγραφέας), Jun-Yong Choi (Συγγραφέας), Sang-Jun Kim (Συγγραφέας), Seung-Il Jeong (Συγγραφέας), Beom-Joon Lee (Συγγραφέας), Kwan-Il Kim (Συγγραφέας), Ran Won (Συγγραφέας), Ji Hoon Jung (Συγγραφέας), Hee Jae Jung (Συγγραφέας), Myungsoo Joo (Συγγραφέας)
• Μορφή: Βιβλίο
• Έκδοση: BMC, 2019-01-01T00:00:00Z.
• Θέματα: article
• Διαθέσιμο Online: Connect to this object online.
• Notes: 10.1186/s12906-018-2422-3; 1472-6882
• Keywords: Formulation of herbal remedies; Asian traditional medicine; Lung inflammation; Nrf2; NF-κB; Other systems of medicine; RZ201-999
• Source: BMC Complementary and Alternative Medicine, Vol 19, Iss 1, Pp 1-12 (2019)

Abstract Background Asian traditional herbal remedies are typically a concoction of a major and several complementary herbs. While balancing out any adverse effect of the major herb, the complementary herbs could dilute the efficacy of the major herb, resulting in a suboptimal therapeutic effect of an herbal remedy. Here, we formulated Chung-Sang (CS) by collating five major herbs, which are used against inflammatory diseases, and tested whether an experimental formula composed of only major herbs is effective in suppressing inflammation without significant side effects. Methods The 50% ethanol extract of CS (eCS) was fingerprinted by HPLC. Cytotoxicity to RAW 264.7 cells was determined by an MTT assay and a flow cytometer. Nuclear NF-κB and Nrf2 were analyzed by western blot. Ubiquitinated Nrf2 was similarly analyzed following immunoprecipitation of Nrf2. Acute lung inflammation and sepsis were induced in C57BL/6 mice. The effects of eCS on lung disease were measured by HE staining of lung sections, a differential cell counting of bronchoalveolar lavage fluid, a myeloperoxidase (MPO) assay, a real-time qPCR, and Kaplan-Meier survival of mice. Results eCS neither elicited cytotoxicity nor reactive oxygen species. While not suppressing NF-κB, eCS activated Nrf2, reduced the ubiquitination of Nrf2, and consequently induced the expression of Nrf2-dependent genes. In an acute lung inflammation mouse model, an intratracheal (i.t.) eCS suppressed neutrophil infiltration, the expression of inflammatory cytokine genes, and MPO activity. In a sepsis mouse model, a single i.t. eCS was sufficient to significantly decrease mouse mortality. Conclusions eCS could suppress severe lung inflammation in mice. This effect seemed to associate with eCS activating Nrf2. Our findings suggest that herbal remedies consisting of only major herbs are worth considering.