In Vitro and In Silico Antioxidant Activity of Novel Peptides Prepared from <i>Paeonia Ostii</i> 'Feng Dan' Hydrolysate

• Main Authors: Min Wang (Author), Cong Li (Author), Haoyu Li (Author), Zibo Wu (Author), Bang Chen (Author), Yibo Lei (Author), Yehua Shen (Author)
• Format: Book
• Published: MDPI AG, 2019-10-01T00:00:00Z.
• Subjects: article
• Online Access: Connect to this object online.

Summary

Antioxidant peptides derived from natural products have superior performance and broader application prospects. In this study, five novel antioxidant peptides were prepared from <i>Paeonia ostii (P. ostii)</i> seed meal, moreover the bioactive and the relationship between structure and properties of antioxidant peptides were elucidated by quantum chemical calculations. The free radical-scavenging activities were used as indexes to purify and concentrate the antioxidant peptides through five proteases and separation techniques. FSAP (Phe-Ser-Ala-Pro), PVETVR (Pro-Val-Glu-Thr-Val-Arg), QEPLLR (Gln-Glu-Pro-Leu-Leu-Arg), EAAY (Glu-Ala-Ala-Tyr) and VLRPPLS (Val-Leu-Arg-Pro-Pro-Leu-Ser) were identified by nano liquid chromatography&#8722;tandem mass spectrometry (LC-MS/MS). In vitro antioxidant activity test, EAAY exhibited the highest 2, 2&#8217;-azino-bis (ABTS) and hydroxyl radical-scavenging activity of 98.5% &#177; 1.1% and 61.9% &#177; 1.3%, respectively (<i>p</i> &lt; 0.01), at 0.5 mg/mL. In silico calculations were carried out using the density functional theory (DFT) with the B3LYP/6-31G* basis set. According to natural bond orbital (NBO) analysis, the bioactivity of free-radical scavenging of the peptides was presumed. Moreover, the antioxidant peptides demonstrated no obvious cytotoxicity to L929 fibroblast cells. Therefore, the peptides from <i>P. ostii</i> seed by-products might potentially have excellent uses in functional foods, nutraceuticals and pharmacological products.

Item Description

2076-3921
10.3390/antiox8100433