Expression of paraoxonase 1 in HEK293 cells and its effect on lactonase and arylesterase activity in samples containing human serum and/or prooxidant or antioxidant agents - a preliminary study

• Main Authors: Anna Bizoń (Author), Agnieszka Piwowar (Author)
• פורמט: ספר
• יצא לאור: Polskie Towarzystwo Farmaceutyczne, 2023-07-01T00:00:00Z.
• נושאים: article
• גישה מקוונת: Connect to this object online.

Expression of recombinant paraoxonase 1 (PON1) in the human embryonic kidney (HEK293F) cell line was evaluated. Two recombinant human PON1 proteins were investigated: PON1-Fc, which has an Fc-tag on its C-terminal, and His-PON1-Fc, which has an Fc-tag on its C-terminal and histidine on its N-termina...