Determination of two capsaicinoids in analgesic transdermal patches using RP-HPLC and UV spectroscopy
Background and objectives: At the present time, a considerable frontier in the administration of therapeutic medications is transdermal drug delivery. Methods: In this study, a rapid, precise, sensitive and selective reversed-phasehigh performance liquid chromatography (RP-HPLC) method has been eval...
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| Main Authors: | , , , , |
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| Format: | Book |
| Published: |
Iranian Society of Pharmacognosy,
2017-11-01T00:00:00Z.
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| Summary: | Background and objectives: At the present time, a considerable frontier in the administration of therapeutic medications is transdermal drug delivery. Methods: In this study, a rapid, precise, sensitive and selective reversed-phasehigh performance liquid chromatography (RP-HPLC) method has been evaluated, developed and validated to separate and quantitate capsaicin and dihydrocapsaicin (main active agents) in analgesic dermal patches produced in Iran. Results: After isolation from laminated adhesive patches, capsaicinoids were analyzed on Lichrospher C18 analytical columns with reversed phase, using a mobile phase composition of methanol and distilled water (70:30 v/v) and without any buffer (pH=6.5). The flow rate was 1 mL/min and the UV detector was operating at 281 nm. The assay was found to be linear over the range of 0.1-1.0 mg/mL. All validation parameters were within the acceptable range. Conclusion: It seems that the developed method was fairly sensitive and reliable in measuring capsaicinoids in commercially available analgesic transdermal patches in Iran. |
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| Item Description: | 2345-4458 2345-5977 |