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ESBL/<i>p</i>AmpC-Producing <i>Escherichia coli</i> Causing Urinary Tract Infections in Non-Related Companion Animals and Humans

Urinary tract infections (UTI) caused by <i>Escherichia coli</i> are frequently diagnosed in humans and companion animals. Extended-spectrum beta-lactamase (ESBL)- and cephalosporinase (<i>p</i>AmpC)-producing <i>Escherichia coli</i> are worldwide-disseminated and...

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Bibliographic Details
Main Authors: Adriana Belas (Author), Cátia Marques (Author), Juliana Menezes (Author), Luís Telo da Gama (Author), Patrícia Cavaco-Silva (Author), Constança Pomba (Author)
Format: Book
Published: MDPI AG, 2022-04-01T00:00:00Z.
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Summary:Urinary tract infections (UTI) caused by <i>Escherichia coli</i> are frequently diagnosed in humans and companion animals. Extended-spectrum beta-lactamase (ESBL)- and cephalosporinase (<i>p</i>AmpC)-producing <i>Escherichia coli</i> are worldwide-disseminated and frequently multidrug-resistant, hence leading to treatment failure and public health concerns. This study aimed to characterize and compare ESBL/<i>p</i>AmpC-producing <i>E. coli</i> strains causing community-acquired UTI in companion animals and non-related humans. Third-generation cephalosporin (3GC)-resistant <i>E. coli</i> (companion animals <i>n</i> = 35; humans <i>n</i> = 85) isolated from patients with UTI were tested against 14 antimicrobials following CLSI guidelines. PCR-based assays were used to detect the major <i>E. coli</i> phylogenetic groups, pathogenicity associated-islands (PAIs), virulence genes, and ESBLs/<i>p</i>AmpC resistance genes. ESBL/<i>p</i>AmpC-producing <i>E. coli</i> isolates were typed by multi-locus sequence typing (MLST) and PCR. <i>E. coli</i> strains from companion animals and humans shared two MDR high-risk clonal lineages: ST131 and ST648. To the best of our knowledge, this study reports the first description of <i>E. coli</i> ST131 clade C1-M27 and the clonal lineage ST131 clade A in humans with community-acquired UTI in Portugal. Considering that companion animals with UTI are generally treated at home by the owners, measures should be implemented to avoid the spread of multidrug-resistant high-risk clones to humans and their household environment.
Item Description:10.3390/antibiotics11050559
2079-6382

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