Anti-Mycoplasma Activity of Daptomycin and Its Use for Mycoplasma Elimination in Cell Cultures of Rickettsiae
Mycoplasma contamination detrimentally affects cellular functions and the growth of intracellular pathogens in cell cultures. Although several mycoplasmacidal agents are commercially available for sterile cell cultures, they are not applicable to rickettsia-infected cells. In our attempt to find an...
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| Main Authors: | , , , , , |
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| Format: | Book |
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MDPI AG,
2019-08-01T00:00:00Z.
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| Summary: | Mycoplasma contamination detrimentally affects cellular functions and the growth of intracellular pathogens in cell cultures. Although several mycoplasmacidal agents are commercially available for sterile cell cultures, they are not applicable to rickettsia-infected cells. In our attempt to find an anti-mycoplasma drug for contaminated rickettsial cultures, we determined the susceptibilities of three common <i>Mycoplasma</i> species to daptomycin. <i>Mycoplasma orale</i> and <i>M. arginini</i> showed low-level resistance to daptomycin (minimum inhibitory concentration, MIC = 2 mg/L), whereas <i>M. hyorhinis</i> was high-level resistant (MIC = 32 mg/L). However, some <i>Mycoplasma</i> isolates developed higher resistance to daptomycin after failed treatments with inadequate doses or durations. An aminoglycoside (gentamicin) was still active against <i>M. hyorhinis</i> and could be used in <i>Orientia</i> cultures. For complete eradication of mycoplasmas in <i>Rickettsia</i> cultures, we recommend a 3-week treatment with daptomycin at 256 mg/L. In contaminated <i>Orientia</i> cultures, daptomycin at 32 mg/L was effective in eradicating <i>M. orale</i>, whereas either gentamicin or amikacin (100 mg/L) was effective in eradicating <i>M. hyorhinis</i>. Unlike each drug alone, the combinations of daptomycin plus clindamycin and/or quinupristin/dalfopristin proved effective in eradicating <i>M. hyorhinis</i>. In summary, our study demonstrated the in vitro anti-mycoplasma activity of daptomycin and its application as a new mycoplasma decontamination method for <i>Rickettsia</i> and <i>Orientia</i> cultures. |
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| Item Description: | 2079-6382 10.3390/antibiotics8030123 |